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NS9803

Resistance of breast and ovarian cancer to cytostatics due to ABC transporters and relation of kinesins to prognosis and prediction

Project goals

It a large cohort of patients we hope to confirm our findings that high expression and polymorfim of MDR1 cause lower effects of taxane and doxorubicin chemotherapy in breast cancer and extend the study to other ABC transporters (MRP1, BCRP). We will extend this study to ovarian cancer, where taxanes belong to most important drugs. The determination of expression in affected lymph nodes may help to predict the risk of relapse it relation to the used chemotherapy. In fresh breast cancer slices we will continue our pilot study relating taxane transport to expression of MDR1 mRNA and protein to indicate whether MDR1 expression may predict drug efflux from tumor cells and thereby possible lower therapeutic effects. We want to confirm the data that KIF14 expression is an efficient prognostic factor in breast cancer, extend the study to ovarian cancer and to investigate its use in prediction and possible relation to taxane effects in vitro.

Keywords

breast cancerovarian cancerABC transportersmultidrug resistanceKIF14proliferationprognosisprediction

Public support

  • Provider

    Ministry of Health

  • Programme

    Departmental Programme of Research and Development - MH II from 2008 to 2011

  • Call for proposals

    VaV pro Ministerstvo zdravotnictví II 1 (SMZ0200801)

  • Main participants

  • Contest type

    VS - Public tender

  • Contract ID

    NS9803-4/2008

Alternative language

  • Project name in Czech

    Resistence nádorů prsu a ovarií na cytostatika vyvolaná ABC transportéry a vztah kinesinů k prognóze a predikci vývoje nemoci

  • Annotation in Czech

    Chceme potvrdit na velkém souboru pacientek náš nález že vysoká exprese a polymorfimus MDR1 působí nižší účinky chemoterapie rakoviny prsu taxany a doxorubicinem a rozšířit tuto studii o expresi a polymorfismy dalších ABC transportérů (MRP1, BCRP). Nověchceme studovat expresi ABC transportérů u rakoviny ovarií, jež by mohla snížit účinky zde nejvýznamnějších cytostatik taxanů a doxorubicinu. Sledováním exprese v postižených uzlinách chceme přispět k predikci rizika relapsu podle použité terapie. V řezech lidských nádorů prsu chceme sledovat transport cytostatik ve vztahu k expresi mRNA a proteinu MDR1 pro potvrzení vztahu mezi nimi a tím ukázat zda lze použít expresi mRNA jako index transportu cytostatik z buněk a tím predikci nižších účinků. Chceme potvrdit význam nového indexu proliferace kinesinu KIF14 pro prognózu rakoviny prsu, zjistit jeho význam pro predikci, zkoumat obě možnosti u rakoviny ovarií a studovat in vitro jeho roli v účincích taxanů jež vyvolávají u buněk identické projevy

Scientific branches

  • R&D category

    NV - Nonindustrial research (Applied research excluded Industrial research)

  • CEP classification - main branch

    EB - Genetics and molecular biology

  • CEP - secondary branch

    FD - Oncology and haematology

  • CEP - another secondary branch

    DN - Environmental impact on health

  • 10603 - Genetics and heredity (medical genetics to be 3)
    10604 - Reproductive biology (medical aspects to be 3)
    10605 - Developmental biology
    10608 - Biochemistry and molecular biology
    10609 - Biochemical research methods
    30101 - Human genetics
    30204 - Oncology
    30205 - Hematology
    30304 - Public and environmental health

Completed project evaluation

  • Provider evaluation

    V - Vynikající výsledky projektu (s mezinárodním významem atd.)

  • Project results evaluation

    Research of new treatment options and diagnosis of tumors is very important. The results suggest ways to use the new options and give cystostatics and new knowledge about monitored the expression of the genes.

Solution timeline

  • Realization period - beginning

    Jul 1, 2008

  • Realization period - end

    Dec 31, 2011

  • Project status

    U - Finished project

  • Latest support payment

    Aug 8, 2011

Data delivery to CEP

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

  • Data delivery code

    CEP12-MZ0-NS-U/09:3

  • Data delivery date

    Oct 31, 2013

Finance

  • Total approved costs

    8,236 thou. CZK

  • Public financial support

    8,236 thou. CZK

  • Other public sources

    0 thou. CZK

  • Non public and foreign sources

    0 thou. CZK

Recognised costs

8 236 CZK thou.

Public support

8 236 CZK thou.

0%


Provider

Ministry of Health

CEP

EB - Genetics and molecular biology

Solution period

01. 07. 2008 - 31. 12. 2011