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NT13092

Heme Catabolic Pathway in Chronic Hepatitis C

Project goals

Heme oxygenase (HMOX), an enzyme induced in response to oxidative stress, catalyzes degradation of pro-oxidative heme to carbon monoxide (CO), iron and biliverdin, which is reduced to bilirubin by biliverdin reductase A (BVRA). Hepatic expression of HMOX is reduced during hepatitis C infection (HCV) in vivo. Biliverdin seems to interfere with HCV replication in vitro. Recently, in the retrospective pilot study, we have found significant association between BVRA expression in PBMC of HCV infected patients prior to antiviral treatment and the response to the therapy. In the proposed prospective clinical trial (registered under ID: NCT01112033), we will study the association of the pre-treatment expression of BVRA in the liver and PBMC with viral clearance, disease progression and treatment response. Analyzing BVRA expression in PBMC might become a promising diagnostic tool. This simple non-invasive test would help to predict which patients will respond to standard antiviral therapy.

Keywords

hepatitis C virusbiliverdin reductasegene expressionoxidative stress

Public support

  • Provider

    Ministry of Health

  • Programme

    Ministry of Health?s Departmental Research and Development Programme III

  • Call for proposals

    VaV pro Ministerstvo zdravotnictví III 3 (SMZ0201201)

  • Main participants

  • Contest type

    VS - Public tender

  • Contract ID

    NT13092-4/2012

Alternative language

  • Project name in Czech

    Katabolická dráha hemu u chronické hepatitidy C

  • Annotation in Czech

    V patogenezi komplikací chronické hepatitidy C hraje roli oxidační stres, indukovaný virem hepatitidy C (HCV). Hemoxygenáza (HMOX), katalyzující degradaci hemu na oxid uhelnatý, železo a biliverdin, který je biliverdinreduktázou A (BVRA) redukován na bilirubin, chrání játra před oxidačním stresem. Dosud bylo prokázáno, že u HCV infekce je exprese HMOX v jaterní tkáni snížena a že biliverdin in vitro interferuje s replikací viru. V naší pilotní retrospektivní studii jsme zjistili, že exprese BVRA v PBMC může predikovat odpověď pacientů s HCV na standardní terapii. V navrhované prospektivní klinické studii (registrované pod ID: NCT01112033) budeme hledat asociaci exprese BVRA před zahájením léčby v jaterní tkáni a PBMC s eliminací viru, histologickým nálezem a s odpovědí na terapii. Pokud studie potvrdí naše předběžná data, analýza exprese BVRA v PBMC by se mohla stát jednoduchým neinvazivním testem, který by mohl přispět k predikci odpovídavosti na protivirovou terapii ještě před jejím zahájením.

Scientific branches

  • R&D category

    NV - Nonindustrial research (Applied research excluded Industrial research)

  • CEP classification - main branch

    FP - Other medical fields

  • CEP - secondary branch

  • CEP - another secondary branch

  • 30106 - Anatomy and morphology (plant science to be 1.6)
    30107 - Medicinal chemistry
    30108 - Toxicology
    30109 - Pathology
    30221 - Critical care medicine and Emergency medicine
    30223 - Anaesthesiology
    30224 - Radiology, nuclear medicine and medical imaging
    30225 - Allergy
    30226 - Rheumatology
    30227 - Geriatrics and gerontology
    30229 - Integrative and complementary medicine (alternative practice systems)
    30230 - Other clinical medicine subjects
    30307 - Nursing
    30308 - Nutrition, Dietetics
    30309 - Tropical medicine
    30310 - Parasitology
    30311 - Medical ethics
    30312 - Substance abuse
    30501 - Forensic science
    30502 - Other medical science

Completed project evaluation

  • Provider evaluation

    V - Vynikající výsledky projektu (s mezinárodním významem atd.)

  • Project results evaluation

    The submitted final report of the reasearch project is high-quality one. Authors found that patients suffering ftom chronic hepatitis C had significantly higher expresion of mRNA biliverdin reductase A of leukocytes compared to heathy controls.

Solution timeline

  • Realization period - beginning

    Apr 1, 2012

  • Realization period - end

    Dec 31, 2015

  • Project status

    U - Finished project

  • Latest support payment

    Mar 20, 2015

Data delivery to CEP

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

  • Data delivery code

    CEP16-MZ0-NT-U/02:1

  • Data delivery date

    Dec 19, 2016

Finance

  • Total approved costs

    4,323 thou. CZK

  • Public financial support

    4,323 thou. CZK

  • Other public sources

    0 thou. CZK

  • Non public and foreign sources

    0 thou. CZK