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QC0048

Molecular and immunoenzymatic diagnosis of selected quarantine, regulated and economical significant plant pathogens.

Project goals

An isolate of ASPV (apple stem pitting virus) will be obtained from IPO-DLO, Wageningen. The isolate will be inoculated and maintained in the indicator plants. RT-PCR will be optimised for the detection of ASPV. Screening of the occurrence of ASPV will be performed in several selected intensive apple and pear orchards in the Czech Republic. According to detailed study, it will be completed a complex method for the detection of ASPV. A PCR technique for the detection of Clavibacter michiganensis subsp. insidiosus (Cmi) in alfalfa tissue will be elaborated. Alfalfa plants will be infected with Cmi. The occurence of Cmi will be tested in seeds, stems and roots respectively of infected plants. A procedure for isolating populations of sporangiaof the causalagent of potatoe wart disease Synchytrium endobioticum from potatoe wart tissue will be devised. After that a PCR based assay for the identification of S. endobioticum races will be developed. The method for the detection of grapevine ne

Keywords

ASPV

Public support

  • Provider

    Ministry of Agriculture

  • Programme

    Scheme I

  • Call for proposals

    SMZE2000I

  • Main participants

    Chmelařský institut s.r.o.
    Národní centrum zemědělského a potravinářského výzkumu, v. v. i.

  • Contest type

    VS - Public tender

  • Contract ID

    —

Alternative language

  • Project name in Czech

    Molekulární a imunoenzymatická diagnostika vybraných karanténních, regulovaných a hospodářsky škodlivých patogenů rostlin

  • Annotation in Czech

    Standardní izolát ASPV (virus vrásčitosti kmene jabloně) bude získán z IPO-DLO ve Wageningenu. Tento izolát bude inokulován a udržován na indikátorových rostlinách. Bude optimalizována metoda RT-PCR pro detekci ASPV a proveden průzkum rozšíření ASPV ve vybraných intenzivních sadech jabloní a hrušní na území ČR. Na základě podrobné studie bude vyhotovena komplexní metodika diagnostiky ASPV. Bude vypracována metodika detekce Clavibacter michiganensis subsp. insidiosus (Cmi) založená na PCR v semenech a rostlinách vojtěšky. Rostliny vojtěšky budou infikovány Cmi a semena sklizená z těchto rostlin budou testována na přítomnost Cmi. Bude optimalizován postup izolace sporangií původce rakoviny brambor Synchytrium endobioticum z nádorů vyvolaných tímto patogenem. Bude vyvinut protokol identifikace jednotlivých ras S. endobioticum využívající PCR. Bude optimalizována metoda detekce nepovirů infikujících révu vinnou (virus roncetu révy vinné - grapevine fanleaf virus GFLV, virus mozaiky huseníku -

Scientific branches

  • R&D category

    NV - Nonindustrial research (Applied research excluded Industrial research)

  • CEP classification - main branch

    GF - Diseases, pests, weeds and plant protection

  • CEP - secondary branch

    EE - Microbiology, virology

  • CEP - another secondary branch

    EB - Genetics and molecular biology

  • 10603 - Genetics and heredity (medical genetics to be 3)
    10604 - Reproductive biology (medical aspects to be 3)
    10605 - Developmental biology
    10606 - Microbiology
    10607 - Virology
    10608 - Biochemistry and molecular biology
    10609 - Biochemical research methods
    30101 - Human genetics
    40106 - Agronomy, plant breeding and plant protection; (Agricultural biotechnology to be 4.4)

Completed project evaluation

  • Provider evaluation

    U - Uspěl podle zadání (s publikovanými či patentovanými výsledky atd.)

  • Project results evaluation

    An isolate of ASPV (apple stem pitting virus) will be obtained from IPO-DLO, Wageningen. The isolate will be inoculated and maintained in the indicator plants. RT-PCR will be optimised for the detection of ASPV. Screening of the occurrence of ASPV will

Solution timeline

  • Realization period - beginning

    Jan 1, 2000

  • Realization period - end

    Jan 1, 2004

  • Project status

    U - Finished project

  • Latest support payment

    —

Data delivery to CEP

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

  • Data delivery code

    CEP/2005/MZE/MZE5QC/U/N/4:1

  • Data delivery date

    Jul 1, 2005

Finance

  • Total approved costs

    21,165 thou. CZK

  • Public financial support

    21,165 thou. CZK

  • Other public sources

    0 thou. CZK

  • Non public and foreign sources

    0 thou. CZK

Recognised costs

21 165 CZK thou.

Public support

21 165 CZK thou.

0%

Provider

Ministry of Agriculture

CEP

GF - Diseases, pests, weeds and plant protection

Solution period

01. 01. 2000 - 01. 01. 2004

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