Alternative (non-GMO) biotechnological approaches for resistance improvement to fungal pathogens in legume crops.
Project goals
Selection agents will be derived from serious pathogens of peas (Fusarium solani f.sp. pisi, Mycosphaerella pinodes), faba bean (F. solani, f. sp. viciae fabae, Ascochyta fabae), and lupin (F. solani, f.sp. lupini, Colletotrichum gleosporioides), aimingto their suitability for utilisation in selections of embryogenic and organogenic cultures in vitro. The three types of selective agents (crude autoclaved filtrate, consequently filtered solution of metabolites, elicitors derived from fungal cell wall components) will be searched for their effects in the in vitro cultures (morphological and physiological changes, content of pathogenesis-related proteins). The reactions in vitro will be compared to the effects of inoculations in vivo. The lines selectedfrom in vitro cultures will be analysed for the content of PR proteins, polymorfism of isoenzymes and DNA and DNA content. The resistance of selected regenerants will be assessed in inoculation tests.
Keywords
hráchboblupinaFusarium solaniMycosphaerella pinodesAscochyta fabaeColletotrichum gleosporioidesselekce in vitroselekční agensembryogenní kulturyorganogenní kulturyPR proteinypolymorfismus isoenzymů a DNAobsah DNA
Public support
Provider
Ministry of Agriculture
Programme
Research program of Ministry of Agriculture 2005 - 2009
Call for proposals
VaV pro Ministerstvo zemědělství 1 (SMZE2005MZE01)
Main participants
—
Contest type
VS - Public tender
Contract ID
39659/2005-13020
Alternative language
Project name in Czech
Alternativní přístupy ve využití biotechnologií pro zvyšování rezistence vybraných luskovin k houbovým patogenům
Annotation in Czech
U závažných patogenů hrachu (Fusarium solani f.sp. pisi, Mycosphaerella pinodes), bobu (F. solani, f. sp. viciae fabae, Ascochyta fabae) a lupiny (F. solani, f.sp. lupini, Colletotrichum gleosporioides) budou odvozena selekční agens vhodná pro selekce vembryogenních a organogenních kulturách in vitro. U tří typů selekčních agens - hrubý autoklávovaný filtrát, postupně filtrovaný roztok metabolitů a elicitory izolované z buněčných stěn hub - bude sledován účinek v kulturách in vitro (morfologické a fyziologické změny, obsah pathogenesis-related (PR) proteinů). Po srovnání reakce in vivo dosažené inokulacemi budou hledány korelační vztahy mezi reakcí in vitro a in vivo. Linie selektované in vitro budou analyzovány na obsah PR proteinů, polymorfismus isoenzymů a DNA a obsah DNA. Odolnost selektovaných regenerantů k patogenům bude hodnocena v inokulačních testech.
Scientific branches
R&D category
ZV - Basic research
CEP classification - main branch
GF - Diseases, pests, weeds and plant protection
CEP - secondary branch
GE - Plant cultivation
CEP - another secondary branch
EB - Genetics and molecular biology
10603 - Genetics and heredity (medical genetics to be 3)
10604 - Reproductive biology (medical aspects to be 3)
10605 - Developmental biology
10608 - Biochemistry and molecular biology
10609 - Biochemical research methods
30101 - Human genetics
40105 - Horticulture, viticulture
40106 - Agronomy, plant breeding and plant protection; (Agricultural biotechnology to be 4.4)
Completed project evaluation
Provider evaluation
U - Uspěl podle zadání (s publikovanými či patentovanými výsledky atd.)
Project results evaluation
Demanding objectives were set out in this project and over its course it was shown that more time is necessary to meet them (a number of publications are so far in print). Despite this, important results were obtained (valuable seed material, a collection
Solution timeline
Realization period - beginning
Feb 1, 2006
Realization period - end
Dec 31, 2009
Project status
U - Finished project
Latest support payment
Apr 30, 2009
Data delivery to CEP
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data delivery code
CEP10-MZE-QG-U/01:1
Data delivery date
Jun 30, 2010
Finance
Total approved costs
4,718 thou. CZK
Public financial support
4,718 thou. CZK
Other public sources
0 thou. CZK
Non public and foreign sources
0 thou. CZK
Basic information
Recognised costs
4 718 CZK thou.
Public support
4 718 CZK thou.
100%
Provider
Ministry of Agriculture
CEP
GF - Diseases, pests, weeds and plant protection
Solution period
01. 02. 2006 - 31. 12. 2009