Analysis of the recombinant proteins by mass spectrometry
Public support
Provider
Královéhradecký kraj
Programme
Hradec Králové Region Innovation fund - Innovation vouchers 2010
Call for proposals
Inovační vouchery 2010 KHK (SKHK201000001)
Main participants
GENERI BIOTECH s.r.o.
Contest type
VS - Public tender
Contract ID
RIF2010 - 8
Alternative language
Project name in Czech
Hmotnostně spektrometrické analýzy rekombinantních proteinů
Annotation in Czech
Předmětem řešení projektu bude hmotnostně spektrometrická analýza (provedená na pracovišti dodavatele) vzorků připravených žadatelem. Cílem projektu bude identifikace a charakterizace vzorků. V rámci tohoto projektu budou prováděny tři typy analýz: 1) Identifikace rekombinantního proteinu a určení jeho primární struktury 2) Identifikace rekombinantního proteinu s detekcí fosforylace a určení místa fosforylace 3) Identifikace rekombinantního proteinu s detekcí glykosylace a následnou charakterizací glykanové části.
Scientific branches
R&D category
VV - Exeperimental development
CEP classification - main branch
EI - Biotechnology and bionics
CEP - secondary branch
—
CEP - another secondary branch
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OECD FORD - equivalent branches <br>(according to the <a href="http://www.vyzkum.cz/storage/att/E6EF7938F0E854BAE520AC119FB22E8D/Prevodnik_oboru_Frascati.pdf">converter</a>)
20801 - Environmental biotechnology<br>20802 - Bioremediation, diagnostic biotechnologies (DNA chips and biosensing devices) in environmental management<br>20803 - Environmental biotechnology related ethics<br>20901 - Industrial biotechnology<br>20902 - Bioprocessing technologies (industrial processes relying on biological agents to drive the process) biocatalysis, fermentation<br>20903 - Bioproducts (products that are manufactured using biological material as feedstock) biomaterials, bioplastics, biofuels, bioderived bulk and fine chemicals, bio-derived novel materials<br>30401 - Health-related biotechnology<br>30402 - Technologies involving the manipulation of cells, tissues, organs or the whole organism (assisted reproduction)<br>30403 - Technologies involving identifying the functioning of DNA, proteins and enzymes and how they influence the onset of disease and maintenance of well-being (gene-based diagnostics and therapeutic interventions [pharmacogenomics, gene-based therapeutics])<br>30404 - Biomaterials (as related to medical implants, devices, sensors)<br>30405 - Medical biotechnology related ethics<br>40401 - Agricultural biotechnology and food biotechnology<br>40402 - GM technology (crops and livestock), livestock cloning, marker assisted selection, diagnostics (DNA chips and biosensing devices for the early/accurate detection of diseases) biomass feedstock production technologies, biopharming<br>40403 - Agricultural biotechnology related ethics
Completed project evaluation
Provider evaluation
U - Uspěl podle zadání (s publikovanými či patentovanými výsledky atd.)
Project results evaluation
Two recomb. proteins produced within the GENERI BIOTECH s.r.o. (GB), hSHBG and sGNGB1 protein. Proteins in different fractions were passed to FVZ UO for SDS-PAGE analysis. In the FVZ UO, protein separation was performed on SDS-PAGE and the isolated proteins were subjected to further analyzes. Protein identification was performed based on the composition of peptide fragments. Positive hSHBG and sHMGB1 were identified. An analysis of post-translational modifications was carried out, where sugar residues were identified. The co-operation procedure between GB and FVZ UO has been optimized for the analysis of recomb. proteins to be used. The results of the hSHBG and sHMGB1 protein analyzes were important for further development of protein production for their commer. use. It is the confirm. of the identity of the proteins and their purity and the confirm. of the presence of glycosylation, which is important for application of proteins produced in mammalian systems. Collabor. will continue.
Solution timeline
Realization period - beginning
Jan 1, 2011
Realization period - end
Mar 31, 2011
Project status
U - Finished project
Latest support payment
Dec 10, 2010
Data delivery to CEP
Confidentiality
C - Předmět řešení projektu podléhá obchodnímu tajemství (§ 504 Občanského zákoníku), ale název projektu, cíle projektu a u ukončeného nebo zastaveného projektu zhodnocení výsledku řešení projektu (údaje P03, P04, P15, P19, P29, PN8) dodané do CEP, jsou upraveny tak, aby byly zveřejnitelné.
Data delivery code
CEP12-KHK-UB-U/01:1
Data delivery date
Oct 23, 2017
Finance
Total approved costs
100 thou. CZK
Public financial support
100 thou. CZK
Other public sources
0 thou. CZK
Non public and foreign sources
0 thou. CZK