Cry 3A ?-endotoxin gene mutagenized for enhanced toxicity to spruce bark beetle in a receptor binding loop

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00020702%3A_____%2F12%3A%230000698" target="_blank" >RIV/00020702:_____/12:#0000698 - isvavai.cz</a>
Result on the web
<a href="http://academicjournals.org/article/article1380799201_Lee%20et%20al.pd" target="_blank" >http://academicjournals.org/article/article1380799201_Lee%20et%20al.pd</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.5897/AJB11.3806" target="_blank" >10.5897/AJB11.3806</a>

Result language
angličtina
Original language name
Cry 3A ?-endotoxin gene mutagenized for enhanced toxicity to spruce bark beetle in a receptor binding loop
Original language description
Bacillus thuringiensis Cry3A gene was redesigned for high expression in Norwegian spruce and the sequence was slightly modified to allow for simple N- and C- terminal deletions and domain II loop 1 exchange for synthetic oligos. Modified Cry3A toxins from 13 variants of the synthetic gene were expressed in Escherichia coli BL21 and their toxicity on spruce bark beetle larvae was tested using spruce bark sandwiches. Mutant toxins with N-terminal deletion and loop 1 duplication showed increased toxicity.
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EI - Biotechnology and bionics
OECD FORD branch
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Project
<a href="/en/project/QH71290" target="_blank" >QH71290: Development of transgenic tissue lines of spruce (Picea abies) showing high toxicity towards bark beatle (Scolytidae) species.</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2012
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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