Norway spruce (Picea abies) genetic transformation with modified Cry3A gene of Bacillus thuringiensis

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F13%3A00396848" target="_blank" >RIV/60077344:_____/13:00396848 - isvavai.cz</a>

Alternative codes found

RIV/60076658:12310/13:43885743

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Norway spruce (Picea abies) genetic transformation with modified Cry3A gene of Bacillus thuringiensis

Original language description

Modified versions of the Cry3A gene of Bacillus thuringiensis (Bt) were transferred into Norway spruce (Picea abies). Both the biolistic approach and Agrobacterium tumefaciens mediated procedure were employed for transformation of embryogenic tissue (ET)cultures. The latter method proved to be more efficient yielding 70 transgenic embryogenic tissue lines compared with 18 lines obtained by biolistics. The modified Cry3A genes were driven by a 35S promoter and the nptII screenable selection marker genewas used in all vectors. The transgenic ETs were molecularly characterized and converted into mature somatic embryos. Germinating embryos formed plantlets which were finally planted into perlite and their Cry3A gene transcription activities were demonstrated by RT-PCR.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2013

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Acta Biochimica Polonica

ISSN

0001-527X

e-ISSN

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Volume of the periodical

60

Issue of the periodical within the volume

3

Country of publishing house

PL - POLAND

Number of pages

6

Pages from-to

395-400

UT code for WoS article

000325237000017

EID of the result in the Scopus database

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