Stable Agrobacterium-mediated transformation of Norway spruce embryogenic tissues using somatic embryo explants
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F11%3A00370255" target="_blank" >RIV/60077344:_____/11:00370255 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Stable Agrobacterium-mediated transformation of Norway spruce embryogenic tissues using somatic embryo explants
Original language description
In conifers and other plants with long reproductive cycles, transformed embryogenic calli can serve as a convenient source of plant material for the testing insecticidal or fungicidal transgene efficiency. In this report, transgenic embryogenic callus was obtained after transformation of somatic embryos of Norway spruce (Picea abies) by Agrobacterium tumefaciens with the gus-intron chimeric gene. The stable integration of transgenes was confirmed by PCR and Southern hybridization. The transformation wassuccessful only with suitable embryogenic line sensitive to Agrobacterium. From nine embryogenic lines tested only one gave transgenic callus
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Result continuities
Project
<a href="/en/project/QH71290" target="_blank" >QH71290: Development of transgenic tissue lines of spruce (Picea abies) showing high toxicity towards bark beatle (Scolytidae) species.</a><br>
Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2011
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Forest Science
ISSN
1212-4834
e-ISSN
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Volume of the periodical
57
Issue of the periodical within the volume
7
Country of publishing house
CZ - CZECH REPUBLIC
Number of pages
4
Pages from-to
277-280
UT code for WoS article
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EID of the result in the Scopus database
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