ER disruption and GFP degradation during non-regenerable transformation of flax with Agrobacterium tumefaciens

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F12%3A33140446" target="_blank" >RIV/61989592:15310/12:33140446 - isvavai.cz</a>

Alternative codes found

RIV/61389030:_____/12:00379091

Result on the web

<a href="http://dx.doi.org/10.1007/s00709-010-0261-2" target="_blank" >http://dx.doi.org/10.1007/s00709-010-0261-2</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00709-010-0261-2" target="_blank" >10.1007/s00709-010-0261-2</a>

Result language

angličtina

Original language name

ER disruption and GFP degradation during non-regenerable transformation of flax with Agrobacterium tumefaciens

Original language description

Flax is considered as plant species susceptible to Agrobacterium-mediated genetic transformation. In this study, stability of flax transformation by Agrobacterium rhizogenes versus Agrobacterium tumefaciens was tested by using combined selection for antibiotic resistance and visual selection of green fluorescent protein (GFP)-fusion reporter targeted to the endoplasmic reticulum (ER). Transformation with A. rhizogenes was stable for over 2 years, whereas transformation by A. tumefaciens resulted in non-regenerable stable transformation which was restricted solely to transgenic callus and lasted only 6-8 weeks. However, shoots regenerated from this callus appeared to be non-transgenic. Importantly, callus and root cells stably transformed with A. rhizogenes showed typical regular organization and dynamics of ER as visualized by GFP-ER marker. On the other hand, callus cells transformed with A. tumefaciens showed disintegrated ER structure and impaired dynamics which was accompanied with

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

<a href="/en/project/ED0007%2F01%2F01" target="_blank" >ED0007/01/01: Centre of the Region Haná for Biotechnological and Agricultural Research</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2012

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Protoplasma: an international journal of cell biology

ISSN

0033-183X

e-ISSN

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Volume of the periodical

249

Issue of the periodical within the volume

1

Country of publishing house

AT - AUSTRIA

Number of pages

11

Pages from-to

53-63

UT code for WoS article

000298754000006

EID of the result in the Scopus database

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