Utilization of SSR markers for identification of gene resources of small-leaved linden reproductive material

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00020702%3A_____%2F20%3AN0000140" target="_blank" >RIV/00020702:_____/20:N0000140 - isvavai.cz</a>
Result on the web
<a href="https://www.vulhm.cz/files/uploads/2020/10/Book-of-Abstracts_Forest-future-2020.pdf" target="_blank" >https://www.vulhm.cz/files/uploads/2020/10/Book-of-Abstracts_Forest-future-2020.pdf</a>
DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Utilization of SSR markers for identification of gene resources of small-leaved linden reproductive material
Original language description
Contribution published in an itnernational conference book of abstracts. Total genomic DNA was extracted by DNA Plant Mini Kit (QIAGEN) from young leaves taken from 377 sampled trees from 38 clones of the model seed orchard. Samples were screened using selected eight polymorphic nuclear microsatellite markers. The obtained data were analysed by statistical programs CERVUS, GenAlEx 6.503. There were detected 79 different alleles at 8 loci in the 377 lime individuals from seed orchard. The obtained results illustrate the utility of the microsatellite loci for assessing spatial patterns of genetic diversity and for individual genotypes identification. 93.6% of the sampled trees could be assigned to the clones represented in the model seed orchard. The identified genetic loci were verified as highly polymorphic and could be further used for clonal identification of lime trees.
Czech name
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Czech description
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Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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