Microsatellite markers as a useful tool for species identification and assessment of genetic diversity of the endangered species Populus nigra L. in the Czech Republic
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00020702%3A_____%2F25%3AN0000080" target="_blank" >RIV/00020702:_____/25:N0000080 - isvavai.cz</a>
Result on the web
<a href="https://www.mdpi.com/1999-4907/16/9/1389" target="_blank" >https://www.mdpi.com/1999-4907/16/9/1389</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3390/f16091389" target="_blank" >10.3390/f16091389</a>
Alternative languages
Result language
angličtina
Original language name
Microsatellite markers as a useful tool for species identification and assessment of genetic diversity of the endangered species Populus nigra L. in the Czech Republic
Original language description
The population size of black poplar (Populus nigra L.), once an important part of floodplain forests in the Czech Republic, has greatly declined due to human activity. In this study, we applied microsatellite (SSR) markers to identify species and assess genetic diversity, with the aim of supporting conservation of this endangered species. A total of 378 poplar trees were analyzed following field surveys. Five diagnostic SSR markers with species-specific alleles for P. deltoides Bartr. ex Marsh. enabled the identification of 39 interspecific hybrids, which were distinguished from native P. nigra. Thirteen SSR loci were used to evaluate genetic diversity among confirmed P. nigra individuals. The results revealed high genetic variation, with 66% of pairwise genotype comparisons differing at all loci. After excluding 45 genetically similar individuals, 292 genetically verified and polymorphic P. nigra trees were selected as potential sources of reproductive material. Genetic differentiation (Fst) was highest between P. nigra and P. deltoides (0.27), and lowest between reference Populus xeuroamericana clones and detected hybrid poplars (0.05) from natural localities. Distinct genetic structures were identified among P. nigra, P. deltoides, and hybrid individuals. These findings provide essential data for the protection, reproduction, and planting of black poplar.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
40102 - Forestry
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Forests
ISSN
1999-4907
e-ISSN
1999-4907
Volume of the periodical
16
Issue of the periodical within the volume
9
Country of publishing house
CH - SWITZERLAND
Number of pages
16
Pages from-to
1389
UT code for WoS article
001580900200001
EID of the result in the Scopus database
2-s2.0-105017228732