Development and characterization of microsatellite markers in black poplar (Populus nigraL.)

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027073%3A_____%2F00%3A00000052" target="_blank" >RIV/00027073:_____/00:00000052 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Development and characterization of microsatellite markers in black poplar (Populus nigraL.)
Original language description
Using an enrichment procedure, we have cloned and sequenced microsatellite loci from black poplar (Populus nigra L.) and developed primers for sequence-tagged microsatellite (STMS) analysis. Twelve primer pairs for dinucleotide repeats produced fragmentsof sufficient quality which were polymorphic in P. nigra.Some of them also showed amplification in other poplar species (P. deltoides, P. tricocarpa, P. tremula, P. tremuloides, P. candicans, and/or P. lasiocarpa). The best nine (GT) (GA) microsatellitemarkers were tested on a set of 23 P. nigra genotypes from all over Europe.The microsatellites were highly polymorphic, with 10 to 19 different alleles per microsatellite locus among these 23 genotypes. Using the other eight marker loci, the level of heterozygosity among the plants was on average 0.71 (range 0.25 to 1.00). The microsatellite markers developed will be useful for screening the genetic diversity in natural populations and gene bank collect
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Publication year
2000
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical
Theoretical and applied genetics. : International journal of breeding research and cell genetics.
ISSN
0040-5752
e-ISSN
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Volume of the periodical
101
Issue of the periodical within the volume
1
Country of publishing house
DE - GERMANY
Number of pages
6
Pages from-to
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UT code for WoS article
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EID of the result in the Scopus database
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