Single-antigen-expressing cell lines are excellent tools for detecting human leukocyte. Antigen-C-reactive antibodies in kidney transplant recipients

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00023001%3A_____%2F05%3A00001288" target="_blank" >RIV/00023001:_____/05:00001288 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Single-antigen-expressing cell lines are excellent tools for detecting human leukocyte. Antigen-C-reactive antibodies in kidney transplant recipients

Original language description

Background. Human leukocyte antigen (HLA)-C is expressed on nucleated cells and platelets in lower levels than HLA-A,B, and its antigens are in linkage disequilibrium with HLA-B antigens. Therefore, HLA-C antibody detection is difficult. The authors questioned whether HLA-C could serve as a target in clinical kidney transplantation using a newly developed assay. Methods. Flow cytometry was performed with sera from patients (n=34) awaiting a kidney retransplant using nine cell lines expressing a single HLA-C antigen (single-antigen lines [SAL]). Results. The SAL were validated with HLA-C-specific alloantisera and human monoclonal antibodies against HLA-A, -B, and -C. The results were in agreement with the specificities previously reported. Exceptions, because of new HLA-C specificities used here, could be explained by epitope sharing between the antigens. With respect to patient sera, 15 of the 34 patients tested (44%) showed serum reactivity toward one or more HLA-C SAL. Conclusions. I

Czech name

Linie buněk exprimujících jediný antigen představují vynikající nástroj pro zjišťování lidských leukocytů. Protilátky reagující na HLA-C u příjemců ledvinového štěpu

Czech description

lidský leukocytární antigen (HLA), protilátky

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

FE - Other fields of internal medicine

OECD FORD branch

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Project

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Continuities

N - Vyzkumna aktivita podporovana z neverejnych zdroju

Publication year

2005

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Transplantation

ISSN

0041-1337

e-ISSN

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Volume of the periodical

79

Issue of the periodical within the volume

9

Country of publishing house

US - UNITED STATES

Number of pages

5

Pages from-to

1268-1272

UT code for WoS article

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EID of the result in the Scopus database

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