Proteomic identification of sperm antigens using serum samples from individuals with and without antisperm antibodies

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11140%2F16%3A10325979" target="_blank" >RIV/00216208:11140/16:10325979 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1111/and.12502" target="_blank" >http://dx.doi.org/10.1111/and.12502</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1111/and.12502" target="_blank" >10.1111/and.12502</a>

Result language

angličtina

Original language name

Proteomic identification of sperm antigens using serum samples from individuals with and without antisperm antibodies

Original language description

The aim of the study was to identify human sperm antigens reacting with polyclonal antisperm antibodies. Protein sperm extracts were subjected to electrofocusing, and next immune reactions (immunoblotting) were carried out with positive for antisperm antibodies and control (not containing antisperm antibodies) serum samples. Proteomic analysis of human sperm proteins resulted in identification of 80 sperm antigens that could be divided into three groups: antigens specific for patients with antisperm antibodies (32), antigens recognised by both infertile patients and control sera (35) and antigens detected by control serum samples only (13). Among antigens specific for infertile patients, there were 12 sperm entities known to be involved in fertilisation process. We have also characterised three protein entities identified only by sera of infertile women. Altogether, the proteomic analysis resulted in identification of 27 sperm entities not reported previously in human sperm proteome. Identified proteins are sperm antigens that could be potentially responsible for immunological infertility. The study also sheds new light on the sperm antigens in aspect of gender specificity. The investigation of human sperm proteome by the use of antisperm antibodies-containing sera of infertile individuals not only may indicate new proteins but also can draft their immunological nature.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

FK - Gynaecology and obstetrics

OECD FORD branch

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Project

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Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2016

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Andrologia

ISSN

0303-4569

e-ISSN

—

Volume of the periodical

48

Issue of the periodical within the volume

6

Country of publishing house

DE - GERMANY

Number of pages

9

Pages from-to

693-701

UT code for WoS article

000380894600012

EID of the result in the Scopus database

2-s2.0-84977559295