Disintegration of Human Sperm and Characterization of Its Antigens

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F11%3A43892503" target="_blank" >RIV/60461373:22330/11:43892503 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Disintegration of Human Sperm and Characterization of Its Antigens

Original language description

For treatment of female immunological infertility and of sensitivity to sperm it is necessary to characterize particular sperm antigens recognized by antibodies. For disintegration of sperm, sonication and chemical or related methods (detergents, benzalkonium chloride, urea, hypoosmotic shock) were used. The characterization was performed by SDS-electrophoresis followed by immublotting using IgG antibodies from 45 sera of infertile females. The serum of an eight-year-old girl was used as a negative control. The use of Triton X-100 seems to be the best way for preparation of sperm protein extract. The interactions of serum IgG antibodies with 68 and 123 kDa sperm proteins were shown to be the most frequently recognized.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CE - Biochemistry

OECD FORD branch

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Project

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Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)<br>S - Specificky vyzkum na vysokych skolach

Publication year

2011

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Chemické listy

ISSN

0009-2770

e-ISSN

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Volume of the periodical

105

Issue of the periodical within the volume

11

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

5

Pages from-to

885-889

UT code for WoS article

000297567400011

EID of the result in the Scopus database

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