Disintegration of Human Sperm and Characterization of Its Antigens
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F11%3A43892503" target="_blank" >RIV/60461373:22330/11:43892503 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Disintegration of Human Sperm and Characterization of Its Antigens
Original language description
For treatment of female immunological infertility and of sensitivity to sperm it is necessary to characterize particular sperm antigens recognized by antibodies. For disintegration of sperm, sonication and chemical or related methods (detergents, benzalkonium chloride, urea, hypoosmotic shock) were used. The characterization was performed by SDS-electrophoresis followed by immublotting using IgG antibodies from 45 sera of infertile females. The serum of an eight-year-old girl was used as a negative control. The use of Triton X-100 seems to be the best way for preparation of sperm protein extract. The interactions of serum IgG antibodies with 68 and 123 kDa sperm proteins were shown to be the most frequently recognized.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CE - Biochemistry
OECD FORD branch
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Result continuities
Project
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Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)<br>S - Specificky vyzkum na vysokych skolach
Others
Publication year
2011
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Chemické listy
ISSN
0009-2770
e-ISSN
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Volume of the periodical
105
Issue of the periodical within the volume
11
Country of publishing house
CZ - CZECH REPUBLIC
Number of pages
5
Pages from-to
885-889
UT code for WoS article
000297567400011
EID of the result in the Scopus database
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