Immunodominant semen proteins I: New patterns of sperm proteins related to female immune infertility

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F13%3A43895536" target="_blank" >RIV/60461373:22330/13:43895536 - isvavai.cz</a>
Result on the web
<a href="http://download.springer.com/static/pdf/219/art%253A10.2478%252Fs11535-013-0214-9.pdf?auth66=1390735354_665cb5621b724b940a71486ef46585a4&ext=.pdf" target="_blank" >http://download.springer.com/static/pdf/219/art%253A10.2478%252Fs11535-013-0214-9.pdf?auth66=1390735354_665cb5621b724b940a71486ef46585a4&ext=.pdf</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.2478/s11535-013-0214-9" target="_blank" >10.2478/s11535-013-0214-9</a>

Result language
angličtina
Original language name
Immunodominant semen proteins I: New patterns of sperm proteins related to female immune infertility
Original language description
Infertility affects approximately 10% of couples at reproductive age. Semen constituents may be potential immunogenic structures for women. The aim of our work is to detect and identify sperm proteins interacting with serum IgG antibodies from women withfertility disorders. The biochemical characterization of sperm antigens was performed using one and two dimensional gel electrophoresis, both of which were followed by immunoblotting. The IgG-binding proteins of interest were identified using mass spectrometry. From the serum pool of 30 infertile women, we detected sperm antigens within a relative molecular mass range between 30-80 kDa with an isoelectric point of 4-7. No antigens were detected using the serum pool from 10 fertile women (control group). Heat shock proteins (HSP 70) were identified as major sperm antigens associated with female immune infertility. Additionally, we report for the first time that alpha-enolase is a significant sperm antigen from the serum pool of infertil
Czech name
—
Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EC - Immunology
OECD FORD branch
—

Project
<a href="/en/project/IAA600110902" target="_blank" >IAA600110902: Influence of ingestion of anthropogenically contaminated soils by heavy metals on changes of biochemical and physiological parameters of rats</a><br>
Continuities
S - Specificky vyzkum na vysokych skolach

Publication year
2013
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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