Detection of donor DNA after heart transplantation: how far could it be affected by blood transfusion and donor chimerism?

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00023001%3A_____%2F07%3A00000783" target="_blank" >RIV/00023001:_____/07:00000783 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Detection of donor DNA after heart transplantation: how far could it be affected by blood transfusion and donor chimerism?

Original language description

The possibility to detect donor DNA in recipient plasma has been discussed as a method to prove organ rejection without a biopsy. Usually, the presence of Y chromosome-specific DNA sequences in female recipients has been used as an example. We have analyzed the presence of part of Y chromosomes in females after heart transplantation. The results suggested that pretransplantation blood transfusion together with cell chimerism of donor organs could be factors that affect detection of donor DNA in recipient plasma. Among females who have undergone transplantation, if the donor organ is chimeric, nested polymerase chain reaction may permit detection of Y chromosome-specific DNA sequences to estimate rejection. In other cases, extremely well-controlled methods using multiple markers need to be developed to avoid the danger of false-positive or false-negative results.

Czech name

Detection of donor DNA after heart transplantation: how far could it be affected by blood transfusion and donor chimerism?

Czech description

The possibility to detect donor DNA in recipient plasma has been discussed as a method to prove organ rejection without a biopsy. Usually, the presence of Y chromosome-specific DNA sequences in female recipients has been used as an example. We have analyzed the presence of part of Y chromosomes in females after heart transplantation. The results suggested that pretransplantation blood transfusion together with cell chimerism of donor organs could be factors that affect detection of donor DNA in recipient plasma. Among females who have undergone transplantation, if the donor organ is chimeric, nested polymerase chain reaction may permit detection of Y chromosome-specific DNA sequences to estimate rejection. In other cases, extremely well-controlled methods using multiple markers need to be developed to avoid the danger of false-positive or false-negative results.

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

FA - Cardiovascular diseases including cardio-surgery

OECD FORD branch

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Project

<a href="/en/project/NR8547" target="_blank" >NR8547: Donor derived free cell DNA as a diagnostic marker of acute rejection in orthotopic heart transplant recipients</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2007

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Transplantation Proceedings

ISSN

0041-1345

e-ISSN

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Volume of the periodical

39

Issue of the periodical within the volume

5

Country of publishing house

US - UNITED STATES

Number of pages

3

Pages from-to

1593-1595

UT code for WoS article

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EID of the result in the Scopus database

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