Protein microarray analysis as a tool for monitoring cellular autoreactivity in type 1 diabetes patients and their relatives

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00023001%3A_____%2F07%3A00001627" target="_blank" >RIV/00023001:_____/07:00001627 - isvavai.cz</a>
Alternative codes found
RIV/61388971:_____/07:00094817 RIV/00064203:_____/07:1626 RIV/00216208:11130/07:1626
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Protein microarray analysis as a tool for monitoring cellular autoreactivity in type 1 diabetes patients and their relatives
Original language description
Background: Autoreactive T cells have a crucial role in type 1 diabetes (T1D) pathogenesis. Objectives: The aim of our study was to monitor the in vitro production of cytokines by peripheral blood mononuclear cells (PBMCs) after stimulation with diabetogenic autoantigens. Subjects: Ten T1D patients (tested at the time of diagnosis and 6 and 12 months later), 10 first-degree relatives of the T1D patients, and 10 controls underwent the study. Methods: PBMCs were stimulated with glutamic acid decarboxylase65 (GAD65) amino acids (a.a.) 247-279, 509-528, and 524-543; proinsulin a.a. 9-23; and tyrosine phosphatase (islet antigen-2)/R2 a.a. 853-872. Interleukin (IL)-2, IL-4, IL-5, IL-6, IL-10, IL-13, interferon (IFN)-γ, tumor necrosis factor β, transforming growth factor β1, and granulocyte colony-stimulating factor (GCSF) were analyzed by protein microarray. Results: Differences in cytokine(s) poststimulatory and mainly in basal production were observed in all groups.
Czech name
Analýza proteinů čipovou technikou (microarray) jako nástroj pro sledování autoreaktivity buněk u diabetiků 1. typu a jejich příbuzných
Czech description
autoreaktivní T-buňka, patogeneze, diabetes 1. typu, cytokiny, mononukleární buňky periferní krve

Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
FB - Endocrinology, diabetology, metabolism, nutrition
OECD FORD branch
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Project
<a href="/en/project/NR8127" target="_blank" >NR8127: DETECTION OF AN ANTI-ISLET T-CELL REACTIVITY IN PATIENTS WITH TYPE 1 DIABETES AND IN THEIR RELATIVES</a><br>
Continuities
N - Vyzkumna aktivita podporovana z neverejnych zdroju

Publication year
2007
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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