Renal activity of Akt kinase in obese Zucker rats

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00023001%3A_____%2F08%3A00001897" target="_blank" >RIV/00023001:_____/08:00001897 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Renal activity of Akt kinase in obese Zucker rats

Original language description

Renal cortical Akt was determined in obese Zucker rats (ZO), a model of DM2, and lean controls (ZL). We also studied expression and phosphorylation of the mammalian target of rapamycin (mTOR) and endothelial NO synthase (eNOS), molecules downstream of Akt in the insulin signaling cascade, and documented modulators of renal injury. Akt activity was measured by a kinase assay with GSK-3 as a substrate. Expression of phosphorylated (active) and total proteins was measured by immunoblotting and immunohistochemistry. Renal Akt activity was increased in ZO as compared to ZL rats, in parallel with progressive hyperinsulinemia. No differences in Akt were observed in the skeletal muscle. Corresponding to increases in Akt activity, ZO rats demonstrated enhancedphosphorylation of renal mTOR. Acute PI3K inhibition with wortmannin (100 mug/kg) attenuated renal Akt and mTOR activities in ZO, but not in ZL rats.

Czech name

Renální aktivita Akt kinázy u obézních potkanů Zucker.

Czech description

Renalní aktivita Akt kinázy, exprese a fosforylace mTOR , aktivita eNO syntázy byly studovány u obézních potkanů kmene Zucker a štíhlých kontrol. Aktivita AKT byla zvýšena u obézních potkanů paralelně s hyperizulinémií. Ve svalech rozdíly v AKT nebyly zjištěny. U obézních potkanů byla zvýšena fosforylace mTOR. Akutní inhibice PI3K wortmaninem snížila renální aktivitu Akt a mTOR u obézních ale nikoliv u štíhlých potkanů.

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

FB - Endocrinology, diabetology, metabolism, nutrition

OECD FORD branch

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Project

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Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2008

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Experimental biology and medicine (Maywood)

ISSN

1535-3702

e-ISSN

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Volume of the periodical

233

Issue of the periodical within the volume

10

Country of publishing house

US - UNITED STATES

Number of pages

11

Pages from-to

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UT code for WoS article

000259729500005

EID of the result in the Scopus database

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