Cytokine gene expression profile in monocytic cells after a co-culture with epithelial cells

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00023001%3A_____%2F12%3A00055950" target="_blank" >RIV/00023001:_____/12:00055950 - isvavai.cz</a>
Result on the web
<a href="http://www.springerlink.com/content/912645q5141645hn/?MUD=MP" target="_blank" >http://www.springerlink.com/content/912645q5141645hn/?MUD=MP</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s12026-012-8338-y" target="_blank" >10.1007/s12026-012-8338-y</a>

Result language
angličtina
Original language name
Cytokine gene expression profile in monocytic cells after a co-culture with epithelial cells
Original language description
Epithelial cells represent an important source of cytokines that may modulate the influx and functions of mononuclear phagocytes. The aim of our study was to characterize changes in the gene expression of selected cytokines in human macrophages co-cultured with respiratory epithelial cells. The A549 alveolar type II-like cell line was co-cultured with THP-1 cells (monocyte/macrophage cell line) in filter-separated mode to avoid their cell-cell contact. At different time-points (0, 4, 8, 12 and 24 h), the cells were harvested separately to evaluate their gene and protein expression (IL-1 beta, IL-6, IL-8, IL-10 and GM-CSF). Quantitative RT-PCR analysis showed prominent changes in the THP-1 cytokine gene expression induced by a co-culture with A549 cells. Fourfold upregulation of mRNA expression has been found in 12 genes and 4-fold downregulation in 5 genes as compared to the unstimulated control sample with a p value smaller than 0.05. The induction of inhibin beta A and IL-1 beta mRNA
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Project
<a href="/en/project/ME%20906" target="_blank" >ME 906: The role of epithelial interactions in final differentiation of mononuclear phagocytes and stem cells</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2012
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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