Conditional knockout of collecting duct bradykinin B2 receptors exacerbates angiotensin II-induced hypertension during high salt intake
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00023001%3A_____%2F16%3A00059588" target="_blank" >RIV/00023001:_____/16:00059588 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11130/16:10323754
Result on the web
<a href="http://www.tandfonline.com/doi/abs/10.3109/10641963.2015.1047945?journalCode=iceh20" target="_blank" >http://www.tandfonline.com/doi/abs/10.3109/10641963.2015.1047945?journalCode=iceh20</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3109/10641963.2015.1047945" target="_blank" >10.3109/10641963.2015.1047945</a>
Alternative languages
Result language
angličtina
Original language name
Conditional knockout of collecting duct bradykinin B2 receptors exacerbates angiotensin II-induced hypertension during high salt intake
Original language description
We elucidated the role of collecting duct kinin B2 receptor (B2R) in the development of salt-sensitivity and angiotensin II (ANG II)-induced hypertension. To this end, we used a Cre-Lox recombination strategy to generate mice lacking Bdkrb2 gene for B2R in the collecting duct (Hoxb7-Cretg/+:Bdkrb2flox/flox). In 3 groups of control (Bdkrb2flox/flox) and 3 groups of UBBdkrb2-/- mice, systolic blood pressure (SBP) responses to high salt intake (4 or 8% NaCl; HS) were monitored by radiotelemetry in comparison with standard salt diet (0.4% NaCl) prior to and during subcutaneous ANG II infusion (1000 ng/min/kg) via osmotic minipumps. High salt intakes alone for 2 weeks did not alter SBP in either strain. ANG II significantly increased SBP equally in control (121 +- 2 to 156 +- 3 mmHg) and UBBdkrb2-/- mice (120 +- 2 to 153 +- 2 mmHg). The development of ANG II-induced hypertension was exacerbated by 4%HS in both control (125 +- 3 to 164 +- 5 mmHg) and UBBdkrb2-/- mice (124 +- 2 to 162 +- 3 mmHg) during 2 weeks. Interestingly, 8%HS caused a more profound and earlier ANG II-induced hypertension in UBBdkrb2-/- (129 +- 2 to 166 +- 3 mmHg) as compared to control (128 +- 2 to 158 +- 2 mmHg) and it was accompanied by body weight loss and increased mortality. In conclusion, targeted inactivation of B2R in the renal collecting duct does not cause salt-sensitivity; however, collecting duct B2R attenuates the hypertensive actions of ANG II under conditions of very high salt intake.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
FA - Cardiovascular diseases including cardio-surgery
OECD FORD branch
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Result continuities
Project
<a href="/en/project/NT14011" target="_blank" >NT14011: The impact of bradykinin B2 receptor gene inactivation in mouse distal tubule on sodium handling in experimental models of hypertension</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2016
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Clinical and experimental hypertension
ISSN
1064-1963
e-ISSN
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Volume of the periodical
38
Issue of the periodical within the volume
1
Country of publishing house
US - UNITED STATES
Number of pages
9
Pages from-to
1-9
UT code for WoS article
000367921300001
EID of the result in the Scopus database
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