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The effects of grafted mesenchymal stem cells labeled with iron oxide or cobalt-zinc-iron nanoparticles on the biological macromolecules of rat brain tissue extracts

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00023001%3A_____%2F17%3A00075975" target="_blank" >RIV/00023001:_____/17:00075975 - isvavai.cz</a>

  • Alternative codes found

    RIV/68378041:_____/17:00476951

  • Result on the web

    <a href="https://www.dovepress.com/the-effects-of-grafted-mesenchymal-stem-cells-labeled-with-iron-oxide--peer-reviewed-fulltext-article-IJN" target="_blank" >https://www.dovepress.com/the-effects-of-grafted-mesenchymal-stem-cells-labeled-with-iron-oxide--peer-reviewed-fulltext-article-IJN</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.2147/IJN.S133156" target="_blank" >10.2147/IJN.S133156</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    The effects of grafted mesenchymal stem cells labeled with iron oxide or cobalt-zinc-iron nanoparticles on the biological macromolecules of rat brain tissue extracts

  • Original language description

    Introduction: Rat mesenchymal stem cells (rMSCs) labeled with 1) poly-l-lysine-coated superparamagnetic iron oxide nanoparticles or 2) silica-coated cobalt-zinc-iron nanoparticles were implanted into the left brain hemisphere of rats, to assess their effects on the levels of oxidative damage to biological macromolecules in brain tissue. Methods: Controls were implanted with unlabeled rMSCs. Animals were sacrificed 24 hours or 4 weeks after the treatment, and the implantation site along with the surrounding tissue was isolated from the brain. At the same intervals, parallel groups of animals were scanned in vivo by magnetic resonance imaging (MRI). The comet assay with enzymes of excision DNA repair (endonuclease III and formamidopyrimidine-DNA glycosylase) was used to analyze breaks and oxidative damage to DNA in the brain tissue. Oxidative damage to proteins and lipids was determined by measuring the levels of carbonyl groups and 15-F-2t-isoprostane (enzyme-linked immunosorbent assay). MRI displayed implants of labeled cells as extensive hypointense areas in the brain tissue. In histological sections, the expression of glial fibrillary acidic protein and CD68 was analyzed to detect astrogliosis and inflammatory response. Results: Both contrast labels caused a similar response in the T-2-weighted magnetic resonance (MR) image and the signal was clearly visible within 4 weeks after implantation of rMSCs. No increase of oxidative damage to DNA, lipids, or proteins over the control values was detected in any sample of brain tissue from the treated animals. Also, immunohistochemistry did not indicate any serious tissue impairment around the graft. Conclusion: Both tested types of nanoparticles appear to be prospective and safe labels for tracking the transplanted cells by MR.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database

  • CEP classification

  • OECD FORD branch

    10608 - Biochemistry and molecular biology

Result continuities

  • Project

    <a href="/en/project/GA16-04340S" target="_blank" >GA16-04340S: Oxide nanomagnets, their properties and interactions with biological systems</a><br>

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Others

  • Publication year

    2017

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    International journal of nanomedicine [online]

  • ISSN

    1178-2013

  • e-ISSN

  • Volume of the periodical

    12

  • Issue of the periodical within the volume

    June 20

  • Country of publishing house

    NZ - NEW ZEALAND

  • Number of pages

    8

  • Pages from-to

    4519-4526

  • UT code for WoS article

    000403712400001

  • EID of the result in the Scopus database

    2-s2.0-85021691952