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Heterogeneity of Human Pancreatic Islet Isolation Around Europe: Results of a Survey Study

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00023001%3A_____%2F20%3A00079143" target="_blank" >RIV/00023001:_____/20:00079143 - isvavai.cz</a>

  • Result on the web

    <a href="https://journals.lww.com/transplantjournal/fulltext/2020/01000/Heterogeneity_of_Human_Pancreatic_Islet_Isolation.36.aspx" target="_blank" >https://journals.lww.com/transplantjournal/fulltext/2020/01000/Heterogeneity_of_Human_Pancreatic_Islet_Isolation.36.aspx</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1097/TP.0000000000002777" target="_blank" >10.1097/TP.0000000000002777</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Heterogeneity of Human Pancreatic Islet Isolation Around Europe: Results of a Survey Study

  • Original language description

    BACKGROUND: Europe is currently the most active region in the field of pancreatic islet transplantation, and many of the leading groups are actually achieving similar good outcomes. Further collaborative advances in the field require the standardization of islet cell product isolation processes, and this work aimed to identify differences in the human pancreatic islet isolation processes within European countries. METHODS: A web-based questionnaire about critical steps, including donor selection, pancreas processing, pancreas perfusion and digestion, islet counting and culture, islet quality evaluation, microbiological evaluation, and release criteria of the product, was completed by isolation facilities participating at the Ninth International European Pancreas and Islet Transplant Association (EPITA) Workshop on Islet-Beta Cell Replacement in Milan. RESULTS: Eleven islet isolation facilities completed the questionnaire. The facilities reported 445 and 53 islet isolations per year over the last 3 years from deceased organ donors and pancreatectomized patients, respectively. This activity resulted in 120 and 40 infusions per year in allograft and autograft recipients, respectively. Differences among facilities emerged in donor selection (age, cold ischemia time, intensive care unit length, amylase concentration), pancreas procurement, isolation procedures (brand and concentration of collagenase, additive, maximum acceptable digestion time), quality evaluation, and release criteria for transplantation (glucose-stimulated insulin secretion tests, islet numbers, and purity). Moreover, even when a high concordance about the relevance of one parameter was evident, thresholds for the acceptance were different among facilities. CONCLUSIONS: The result highlighted the presence of a heterogeneity in the islet cell product process and product release criteria.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database

  • CEP classification

  • OECD FORD branch

    30213 - Transplantation

Result continuities

  • Project

  • Continuities

    N - Vyzkumna aktivita podporovana z neverejnych zdroju

Others

  • Publication year

    2020

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Transplantation

  • ISSN

    0041-1337

  • e-ISSN

  • Volume of the periodical

    104

  • Issue of the periodical within the volume

    1

  • Country of publishing house

    US - UNITED STATES

  • Number of pages

    7

  • Pages from-to

    190-196

  • UT code for WoS article

    000528036600041

  • EID of the result in the Scopus database

    2-s2.0-85077223375