Heterogeneity of Human Pancreatic Islet Isolation Around Europe: Results of a Survey Study
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00023001%3A_____%2F20%3A00079143" target="_blank" >RIV/00023001:_____/20:00079143 - isvavai.cz</a>
Result on the web
<a href="https://journals.lww.com/transplantjournal/fulltext/2020/01000/Heterogeneity_of_Human_Pancreatic_Islet_Isolation.36.aspx" target="_blank" >https://journals.lww.com/transplantjournal/fulltext/2020/01000/Heterogeneity_of_Human_Pancreatic_Islet_Isolation.36.aspx</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1097/TP.0000000000002777" target="_blank" >10.1097/TP.0000000000002777</a>
Alternative languages
Result language
angličtina
Original language name
Heterogeneity of Human Pancreatic Islet Isolation Around Europe: Results of a Survey Study
Original language description
BACKGROUND: Europe is currently the most active region in the field of pancreatic islet transplantation, and many of the leading groups are actually achieving similar good outcomes. Further collaborative advances in the field require the standardization of islet cell product isolation processes, and this work aimed to identify differences in the human pancreatic islet isolation processes within European countries. METHODS: A web-based questionnaire about critical steps, including donor selection, pancreas processing, pancreas perfusion and digestion, islet counting and culture, islet quality evaluation, microbiological evaluation, and release criteria of the product, was completed by isolation facilities participating at the Ninth International European Pancreas and Islet Transplant Association (EPITA) Workshop on Islet-Beta Cell Replacement in Milan. RESULTS: Eleven islet isolation facilities completed the questionnaire. The facilities reported 445 and 53 islet isolations per year over the last 3 years from deceased organ donors and pancreatectomized patients, respectively. This activity resulted in 120 and 40 infusions per year in allograft and autograft recipients, respectively. Differences among facilities emerged in donor selection (age, cold ischemia time, intensive care unit length, amylase concentration), pancreas procurement, isolation procedures (brand and concentration of collagenase, additive, maximum acceptable digestion time), quality evaluation, and release criteria for transplantation (glucose-stimulated insulin secretion tests, islet numbers, and purity). Moreover, even when a high concordance about the relevance of one parameter was evident, thresholds for the acceptance were different among facilities. CONCLUSIONS: The result highlighted the presence of a heterogeneity in the islet cell product process and product release criteria.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30213 - Transplantation
Result continuities
Project
—
Continuities
N - Vyzkumna aktivita podporovana z neverejnych zdroju
Others
Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Transplantation
ISSN
0041-1337
e-ISSN
—
Volume of the periodical
104
Issue of the periodical within the volume
1
Country of publishing house
US - UNITED STATES
Number of pages
7
Pages from-to
190-196
UT code for WoS article
000528036600041
EID of the result in the Scopus database
2-s2.0-85077223375