Elevated Circulating Stem Cells Level is Observed One Month After Implantation of Carmat Bioprosthetic Total Artificial Heart
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00023001%3A_____%2F21%3A00081750" target="_blank" >RIV/00023001:_____/21:00081750 - isvavai.cz</a>
Result on the web
<a href="https://link.springer.com/content/pdf/10.1007/s12015-021-10270-3.pdf" target="_blank" >https://link.springer.com/content/pdf/10.1007/s12015-021-10270-3.pdf</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s12015-021-10270-3" target="_blank" >10.1007/s12015-021-10270-3</a>
Alternative languages
Result language
angličtina
Original language name
Elevated Circulating Stem Cells Level is Observed One Month After Implantation of Carmat Bioprosthetic Total Artificial Heart
Original language description
The Aeson® total artificial heart (A-TAH) has been developed as a total heart replacement for patients at risk of death from biventricular failure. We previously described endothelialization of the hybrid membrane inside A-TAH probably at the origin of acquired hemocompatibility. We aimed to quantify vasculogenic stem cells in peripheral blood of patients with long-term A-TAH implantation. Four male adult patients were included in this study. Peripheral blood mononuclear cells were collected before A-TAH implantation (T0) and after implantation at one month (T1), between two and five months (T2), and then between six and twelve months (T3). Supervised analysis of flow cytometry data confirmed the presence of the previously identified Lin(-)CD133(+)CD45(-) and Lin(-)CD34(+) with different CD45 level intensities. Lin(-)CD133(+)CD45(-), Lin(-)CD34(+)CD45(-) and Lin(-)CD34(+)CD45(+) were not modulated after A-TAH implantation. However, we demonstrated a significant mobilization of Lin(-)CD34(+)CD45(dim) (p = 0.01) one month after A-TAH implantation regardless of the expression of CD133 or c-Kit. We then visualized data for the resulting clusters on a uniform manifold approximation and projection (UMAP) plot showing all single cells of the live Lin(-) and CD34(+) events selected from down sampled files concatenated at T0 and T1. The three clusters upregulated at T1 are CD45(dim) clusters, confirming our results. In conclusion, using a flow cytometry approach, we demonstrated in A-TAH-transplanted patients a significant mobilization of Lin(-)CD34(+)CD45(dim) in peripheral blood one month after A-TAH implantation. Using a flow cytometry approach, we demonstrated in A-TAH transplanted patients a significant mobilization of Lin(-)CD34(+)CD45(dim) in peripheral blood one month after A-TAH implantation. This cell population could be at the origin of newly formed endothelial cells on top of hybrid membrane in Carmat bioprosthetic total artificial heart.
Czech name
—
Czech description
—
Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
30213 - Transplantation
Result continuities
Project
—
Continuities
N - Vyzkumna aktivita podporovana z neverejnych zdroju
Others
Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Stem cell reviews and reports
ISSN
2629-3269
e-ISSN
—
Volume of the periodical
17
Issue of the periodical within the volume
6
Country of publishing house
US - UNITED STATES
Number of pages
6
Pages from-to
2332-2337
UT code for WoS article
000705760700001
EID of the result in the Scopus database
2-s2.0-85116591868