In Vitro P-31 MR Chemical Shifts of In Vivo-Detectable Metabolites at 3T as a Basis Set for a Pilot Evaluation of Skeletal Muscle and Liver P-31 Spectra with LCModel Software

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00023001%3A_____%2F21%3A00082003" target="_blank" >RIV/00023001:_____/21:00082003 - isvavai.cz</a>

Result on the web

<a href="https://www.mdpi.com/1420-3049/26/24/7571" target="_blank" >https://www.mdpi.com/1420-3049/26/24/7571</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/molecules26247571" target="_blank" >10.3390/molecules26247571</a>

Result language

angličtina

Original language name

In Vitro P-31 MR Chemical Shifts of In Vivo-Detectable Metabolites at 3T as a Basis Set for a Pilot Evaluation of Skeletal Muscle and Liver P-31 Spectra with LCModel Software

Original language description

Most in vivo P-31 MR studies are realized on 3T MR systems that provide sufficient signal intensity for prominent phosphorus metabolites. The identification of these metabolites in the in vivo spectra is performed by comparing their chemical shifts with the chemical shifts measured in vitro on high-field NMR spectrometers. To approach in vivo conditions at 3T, a set of phantoms with defined metabolite solutions were measured in a 3T whole-body MR system at 7.0 and 7.5 pH, at 37 degrees C. A free induction decay (FID) sequence with and without H-1 decoupling was used. Chemical shifts were obtained of phosphoenolpyruvate (PEP), phosphatidylcholine (PtdC), phosphocholine (PC), phosphoethanolamine (PE), glycerophosphocholine (GPC), glycerophosphoetanolamine (GPE), uridine diphosphoglucose (UDPG), glucose-6-phosphate (G6P), glucose-1-phosphate (G1P), 2,3-diphosphoglycerate (2,3-DPG), nicotinamide adenine dinucleotide (NADH and NAD(+)), phosphocreatine (PCr), adenosine triphosphate (ATP), adenosine diphosphate (ADP), and inorganic phosphate (Pi). The measured chemical shifts were used to construct a basis set of P-31 MR spectra for the evaluation of P-31 in vivo spectra of muscle and the liver using LCModel software (linear combination model). Prior knowledge was successfully employed in the analysis of previously acquired in vivo data.

Czech name

—

Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10608 - Biochemistry and molecular biology

Project

<a href="/en/project/8J18AT023" target="_blank" >8J18AT023: Improvement in liver disease diagnosis using advanced quantitative methods of magnetic resonance</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2021

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Molecules

ISSN

1420-3049

e-ISSN

—

Volume of the periodical

26

Issue of the periodical within the volume

24

Country of publishing house

CH - SWITZERLAND

Number of pages

15

Pages from-to

"art. no. 7571"

UT code for WoS article

000736745800001

EID of the result in the Scopus database

2-s2.0-85121489232