Origin of the (31) P MR signal at 5.3 ppm in patients with critical limb ischemia
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00023001%3A_____%2F20%3A00079645" target="_blank" >RIV/00023001:_____/20:00079645 - isvavai.cz</a>
Result on the web
<a href="https://onlinelibrary.wiley.com/doi/abs/10.1002/nbm.4295" target="_blank" >https://onlinelibrary.wiley.com/doi/abs/10.1002/nbm.4295</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/nbm.4295" target="_blank" >10.1002/nbm.4295</a>
Alternative languages
Result language
angličtina
Original language name
Origin of the (31) P MR signal at 5.3 ppm in patients with critical limb ischemia
Original language description
An unknown intense signal (P(un) ) with a mean chemical shift of 5.3 ppm was observed in (31) P MR spectra from the calf muscles of patients with the diabetic foot syndrome. The aim of the study was to identify the origin of this signal and its potential as a biomarker of muscle injury. Calf muscles of 68 diabetic patients (66.3 ± 8.6 years; body mass index = 28.2 ± 4.3 kg/m(2) ) and 12 age-matched healthy controls were examined by (dynamic) (31) P MRS (3 T system, (31) P/(1) H coil). Phantoms (glucose-1-phosphate, P(i) and PCr) were measured at pH values of 7.05 and 7.51. At rest, P(un) signals with intensities higher than 50% of the P(i) intensity were observed in 10 of the 68 examined diabetic subjects. We tested two hypothetical origins of the P(un) signal: (1) phosphorus from phosphoesters and (2) phosphorus from extra- and intracellular alkaline phosphate pools. 2,3-diphosphoglycerate and glucose-1-phosphate are the only phosphoesters with signals in the chemical shift region close to 5.3 ppm. Both compounds can be excluded: 2,3-diphosphoglycerate due to the missing second signal component at 6.31 ppm; glucose-1-phosphate because its chemical shifts are about 0.2 ppm downfield from the P(i) signal (4.9 ppm). If the P(un) signal is from phosphate, it represents a pH value of 7.54 ± 0.05. Therefore, it could correspond to signals of P(i) in mitochondria. However, patients with critical limb ischemia have rather few mitochondria and so the P(un) signal probably originates from interstitia. Our data suggest that the increased P(un) signal observed in patients with the diabetic foot syndrome is a biomarker of severe muscular damage.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30224 - Radiology, nuclear medicine and medical imaging
Result continuities
Project
<a href="/en/project/NV16-27262A" target="_blank" >NV16-27262A: A comparison of the impact of autologous stem cells therapy and standard revascularization on tissue oxygen supply and course of the diabetic foot</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
NMR in biomedicine
ISSN
0952-3480
e-ISSN
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Volume of the periodical
33
Issue of the periodical within the volume
6
Country of publishing house
US - UNITED STATES
Number of pages
10
Pages from-to
"e4295"
UT code for WoS article
000534219200003
EID of the result in the Scopus database
2-s2.0-85081722389