Clinical and( 31)P magnetic resonance spectroscopy characterization of patients with critical limb ischemia before and after autologous cell therapy
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00023001%3A_____%2F19%3A00078318" target="_blank" >RIV/00023001:_____/19:00078318 - isvavai.cz</a>
Result on the web
<a href="https://www.biomed.cas.cz/physiolres/pdf/2019/68_559.pdf" target="_blank" >https://www.biomed.cas.cz/physiolres/pdf/2019/68_559.pdf</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.33549/physiolres.934107" target="_blank" >10.33549/physiolres.934107</a>
Alternative languages
Result language
angličtina
Original language name
Clinical and( 31)P magnetic resonance spectroscopy characterization of patients with critical limb ischemia before and after autologous cell therapy
Original language description
Autologous cell therapy (ACT) is a new treatment method for diabetic patients with critical limb ischemia (CLI) not eligible for standard revascularization. After intramuscular injection of bone marrow-derived mononuclear cells local arteriogenesis in the ischemic tissue occurs. Studies assessing visualization of this therapeutic vasculogenesis after ACT by novel imaging techniques are lacking. The aim of our study was to assess the effect of ACT on possible metabolic changes and perfusion of critically ischemic limbs using P- 31 magnetic resonance spectroscopy (P-31 MRS) and its possible correlation with changes of transcutaneous oxygen pressure (TcPO2). Twenty-one patients with diabetes and no-option CLI treated by ACT in our foot clinic over 8 years were included in the study. TcPO2 as well as rest (phosphocreatine, adenosine triphosphate and inorganic phosphate) and dynamic (mitochondria) capacity and phosphocreatine recovery time) P- 31-MRS parameters were evaluated at baseline and 3 months after cell treatment. TcPO2 increased significantly after 3 months compared with baseline (from 22.4 +/- 8.2 to 37.6 +/- 13.3 mm Hg, p=0.0002). Rest and dynamic P- 31 MRS parameters were not significantly different after ACT in comparison with baseline values. Our study showed a significant increase of TcPO2 on the dorsum of the foot after ACT. We did not observe any changes of rest or dynamic P- 31 MRS parameters in the area of the proximal calf where the cell suspension has been injected into.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30202 - Endocrinology and metabolism (including diabetes, hormones)
Result continuities
Project
<a href="/en/project/NV16-27262A" target="_blank" >NV16-27262A: A comparison of the impact of autologous stem cells therapy and standard revascularization on tissue oxygen supply and course of the diabetic foot</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Physiological research
ISSN
0862-8408
e-ISSN
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Volume of the periodical
68
Issue of the periodical within the volume
4
Country of publishing house
CZ - CZECH REPUBLIC
Number of pages
8
Pages from-to
559-566
UT code for WoS article
000483374200004
EID of the result in the Scopus database
2-s2.0-85071708639