The effects of immunosuppressive drugs on the characteristics and functional properties of bone marrow-derived stem cells isolated from patients with diabetes mellitus and peripheral arterial disease
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00023001%3A_____%2F23%3A00084087" target="_blank" >RIV/00023001:_____/23:00084087 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11310/23:10466412 RIV/00216208:11110/23:10466412
Result on the web
<a href="https://www.mdpi.com/2227-9059/11/7/1872" target="_blank" >https://www.mdpi.com/2227-9059/11/7/1872</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3390/biomedicines11071872" target="_blank" >10.3390/biomedicines11071872</a>
Alternative languages
Result language
angličtina
Original language name
The effects of immunosuppressive drugs on the characteristics and functional properties of bone marrow-derived stem cells isolated from patients with diabetes mellitus and peripheral arterial disease
Original language description
Background: Diabetic patients (DPs) with foot ulcers can receive autologous cell therapy (ACT) as a last therapeutic option. Even DPs who have undergone organ transplantation and are using immunosuppressive (IS) drugs can be treated by ACT. The aim of our study was to analyze the effects of IS drugs on the characteristics of bone marrow-derived stem cells (BM-MSCs). Methods: The cells were isolated from the bone marrow of DPs, cultivated for 14-18 days, and phenotypically characterized using flow cytometry. These precursor cells were cultured in the presence of various IS drugs. The impact of IS drugs on metabolic activity was measured using a WST-1 assay, and the expression of genes for immunoregulatory molecules was detected through RT-PCR. Cell death was analyzed through the use of flow cytometry, and the production of cytokines was determined by ELISA. Results: The mononuclear fraction of cultured cells contained mesenchymal stem cells (CD45(-)CD73(+)CD90(+)CD105(+)), myeloid angiogenic cells (CD45(+)CD146(-)), and endothelial colony-forming cells (CD45(-)CD146(+)). IS drugs inhibited metabolic activity, the expression of genes for immunoregulatory molecules, the production of cytokines, and the viability of the cells. Conclusions: The results indicate that IS drugs in a dose-dependent manner had a negative impact on the properties of BM-MSCs used to treat ischemic diabetic foot ulcers, and that these drugs could affect the therapeutic potential of BM-MSCs.
Czech name
—
Czech description
—
Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
30202 - Endocrinology and metabolism (including diabetes, hormones)
Result continuities
Project
<a href="/en/project/LX22NPO5104" target="_blank" >LX22NPO5104: National Institute for Research of Metabolic and Cardiovascular Diseases</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2023
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Biomedicines
ISSN
2227-9059
e-ISSN
2227-9059
Volume of the periodical
11
Issue of the periodical within the volume
7
Country of publishing house
CH - SWITZERLAND
Number of pages
14
Pages from-to
"art. no. 1872"
UT code for WoS article
001037991500001
EID of the result in the Scopus database
2-s2.0-85175113930