Female prediabetic rats are protected from vascular dysfunction: the role of nitroso and sulfide signaling

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00023001%3A_____%2F24%3A00085221" target="_blank" >RIV/00023001:_____/24:00085221 - isvavai.cz</a>

Result on the web

<a href="https://biolres.biomedcentral.com/articles/10.1186/s40659-024-00575-1" target="_blank" >https://biolres.biomedcentral.com/articles/10.1186/s40659-024-00575-1</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1186/s40659-024-00575-1" target="_blank" >10.1186/s40659-024-00575-1</a>

Result language

angličtina

Original language name

Female prediabetic rats are protected from vascular dysfunction: the role of nitroso and sulfide signaling

Original language description

BackgroundThe activity of perivascular adipose tissue (PVAT), a specific deposit of adipose tissue surrounding blood vessels, could contribute to sex differences in vascular tone control, particularly in dyslipidemic disorders; however, the mutual associations remain unclear. This study aimed to evaluate the relationships among sex, PVAT and vascular function in Wistar and hereditary hypertriglyceridemic (HTG) rats. Vasoactive responses of the isolated thoracic aorta with preserved or removed PVAT were compared in adult male and female Wistar and HTG rats, and the roles of nitric oxide (NO), hydrogen sulfide (H2S), cyclooxygenase (COX) and inflammatory signaling in vascular function were monitored in females.ResultsHTG rats were hypertensive, but females less than males. Increased 2-h glycemia was observed in HTG rats regardless of sex; however, HTG females exhibited better glucose utilization than males did. Females, independent of strain, had better preserved endothelial function than males did. PVAT inhibited endothelium-dependent relaxation in all the rats except HTG females. In HTG males, pathologically increased aortic contractility was noted; however, in HTG females, the contractile responses were lower, thus approaching physiological levels despite the pro-contractile action of COX products. In HTG females, NO contributed to endothelial function to a lesser extent than it did in controls, but the presence of PVAT eliminated this difference, which corresponded with increased NO synthase activity. Although increased protein expression of several proinflammatory factors (TNF alpha, IL-6, iNOS, and Nf kappa B) was confirmed in the aortic and PVAT tissue of HTG females, the protein expression of factors regulating the adhesion and infiltration of monocytes (ICAM-1 and MCP-1) was decreased in PVAT. Moreover, in HTG females, unlike in controls, H2S produced by PVAT did not inhibit endothelial relaxation, and regardless of PVAT, endogenous H2S had beneficial anticontractile effects, which were associated with increased protein expression of H2S-producing enzymes in both aortic and PVAT tissues.ConclusionsDespite increased inflammation and the pathological impact of cyclooxygenase signaling in female HTG rats, protective vasoactive mechanisms associated with milder hypertension and improved endothelial function and contractility linked to PVAT activity were triggered. Sulfide and nitroso signaling represent important compensatory vasoactive mechanisms against hypertriglyceridemia-associated metabolic disorders and may be promising therapeutic targets in prediabetic females.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10608 - Biochemistry and molecular biology

Project

<a href="/en/project/LUASK22012" target="_blank" >LUASK22012: The role of inflammation in the development of cardiovascular complications associated with metabolic syndrome and prediabetes</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2024

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

BIOLOGICAL RESEARCH

ISSN

0716-9760

e-ISSN

0717-6287

Volume of the periodical

57

Issue of the periodical within the volume

1

Country of publishing house

CL - CHILE

Number of pages

17

Pages from-to

"art. no. 91"

UT code for WoS article

001362582000001

EID of the result in the Scopus database

2-s2.0-85210142618