Accumulation of chlorinated paraffins in adipocytes is determined by cellular lipid content and chlorination level

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00023001%3A_____%2F25%3A00085411" target="_blank" >RIV/00023001:_____/25:00085411 - isvavai.cz</a>

Alternative codes found

RIV/60461373:22330/25:43930277

Result on the web

<a href="https://link.springer.com/content/pdf/10.1007/s00204-024-03956-3.pdf" target="_blank" >https://link.springer.com/content/pdf/10.1007/s00204-024-03956-3.pdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00204-024-03956-3" target="_blank" >10.1007/s00204-024-03956-3</a>

Result language

angličtina

Original language name

Accumulation of chlorinated paraffins in adipocytes is determined by cellular lipid content and chlorination level

Original language description

Chlorinated paraffins (CPs) are environmental pollutants extensively used in industries. While the use of short-chain chlorinated paraffins (SCCPs) has been restricted since 2017, the use of medium-chain chlorinated paraffins (MCCPs) has risen as their replacement. Due to lipophilic character, it can be expected that CPs enter the cells; however, the in vitro accumulation potential of CPs remains poorly understood. In this study, we aimed to explore the ability of SCCPs and MCCPs to accumulate in fat cells. We utilized an in vitro model of mouse 3T3-L1 preadipocytes and adipocytes. Using gas chromatography coupled with high-resolution mass spectrometry operated in negative chemical ionization mode, we determined the intracellular amounts of CPs. These compounds accumulated at rates of 8.5 +/- 0.1 mu g/gcells/h for SCCPs and 7.8 +/- 0.3 mu g/gcells/h for MCCPs when an initial concentration of 120 ng/ml was present in the medium. This rate increased approximately tenfold when the concentration of CPs was raised to 1200 ng/ml. CPs content in adipocytes steadily increased over 5 days, whereas preadipocytes accumulated 15-20 times less CPs. This highlights the importance of cellular lipid content, which was about 12 times higher in adipocytes. Furthermore, we found that the level of chlorine content in the CPs molecules significantly influenced their accumulation. Our results demonstrate that MCCPs exhibit a similar accumulation potential to SCCPs, with lipid content playing a crucial role. As with SCCPs, restrictions on the use of MCCPs in industry should be considered to mitigate their environmental and health impacts.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

30108 - Toxicology

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

V - Vyzkumna aktivita podporovana z jinych verejnych zdroju

Publication year

2025

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Archives of toxicology

ISSN

0340-5761

e-ISSN

1432-0738

Volume of the periodical

99

Issue of the periodical within the volume

3

Country of publishing house

DE - GERMANY

Number of pages

15

Pages from-to

"S1117"-1131

UT code for WoS article

001392752500001

EID of the result in the Scopus database

2-s2.0-85217196072