Is repeated cypermethrin fumigation dangerous for the mitochondrial DNA in dry insect samples?
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00023272%3A_____%2F18%3A10134054" target="_blank" >RIV/00023272:_____/18:10134054 - isvavai.cz</a>
Result on the web
<a href="http://www.aemnp.eu/PDF/58_2/58_2_609.pdf" target="_blank" >http://www.aemnp.eu/PDF/58_2/58_2_609.pdf</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.2478/aemnp-2018-0051" target="_blank" >10.2478/aemnp-2018-0051</a>
Alternative languages
Result language
angličtina
Original language name
Is repeated cypermethrin fumigation dangerous for the mitochondrial DNA in dry insect samples?
Original language description
Entomological collections are the target of various insect pests, e.g. carpet beetles (Dermestidae) and booklice (Psocoptera) which can damage and completely destroy dry specimens in a relatively short time. Collections in the National Museum, Czech Republic (NMP) including the entomological ones are protected by fumigation using commercially available smoke shells 'Cytrol Super SG'; fumigation is performed twice a year. The active insecticidal substance of these smoke shells is cypermethrin (6.25%). We tested whether the repeated cypermethrin fumigation of the NMP entomological collections negatively affects the quality of mitochondrial DNA in dry specimens and prevents the subsequent use of these samples for molecular analyses required for identifi cation, taxonomy, systematics, and phylogenetic studies. We used 32 freshly fixed specimens of the flower chafer Oxythyrea funesta (Poda von Neuhaus, 1761) and 32 freshly fixed specimens of the brown-tailed cockroach Supella longipalpa (Fabricius, 1798). One half of specimens of both species was stored outside NMP and not fumigated (negative control), and the other half was deposited in collection hall with the NMP insect collection and directly exposed to the fumigation. Subsequently, all specimens were processed in a molecular laboratory under a standardized protocol using one leg as the source tissue after each fumigation, and the 658 bp long barcoding region of the cytochrome oxidase I (cox1) as the testing gene fragment. Results of the PCR product electrophoresis and the sequences acquired confirmed that the repeated fumigation had no negative effect on tested samples.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10616 - Entomology
Result continuities
Project
<a href="/en/project/DG16P02B038" target="_blank" >DG16P02B038: A methodology for the determination of zoological collection specimens on the basis of DNA analysis and the management of tissue samples collection</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Acta Entomologica Musei Nationalis Pragae
ISSN
0374-1036
e-ISSN
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Volume of the periodical
58
Issue of the periodical within the volume
2
Country of publishing house
CZ - CZECH REPUBLIC
Number of pages
6
Pages from-to
609-614
UT code for WoS article
000455962400026
EID of the result in the Scopus database
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