How to store a beetle larva? Comparing temporal effects of common fluid preservation methods on color, shape, and DNA quality

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41330%2F24%3A97967" target="_blank" >RIV/60460709:41330/24:97967 - isvavai.cz</a>

Result on the web

<a href="https://academic.oup.com/jme/advance-article/doi/10.1093/jme/tjad154/7471351" target="_blank" >https://academic.oup.com/jme/advance-article/doi/10.1093/jme/tjad154/7471351</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1093/jme/tjad154" target="_blank" >10.1093/jme/tjad154</a>

Result language

angličtina

Original language name

How to store a beetle larva? Comparing temporal effects of common fluid preservation methods on color, shape, and DNA quality

Original language description

Proper fixing and long-term preservation of entomological evidence are essential in collections and research and crucial in applied fields such as forensic entomology. Incorrectly stored samples may lose important morphological features over time, rendering molecular analyses exceedingly difficult. The most effective method for preserving soft samples such as larvae is fluid preservation. It uses a combination of a wide range of fixatives and storage fluids. However, very little comparative work has been done to determine the effects of long-term storage on sample quality in terms of color, shape, and DNA stability. Moreover, the current golden standard in forensic entomology has been tailored for age estimation of larvae of Diptera, which differ from larvae of Coleoptera in morphology and subsequently in applied methods. We compared the effects of combinations of 6 commonly used fixatives and 6 commonly used storage fluids on midsized larvae of the forensically important beetle, Necrodes littoralis (Linnaeus, 1758), in terms of color, shape, and suitability for DNA analyses over a 2-yr period. We were looking for combinations that can preserve specimens in a satisfactory state, can be used on a regular basis, do not require advanced protection or skills of the personnel, and are not toxic or too harmful to the environment. We found not only several methods that scored significantly better in the tested parameters compared with the golden standard but also several common methods that should be avoided. The effects of agents on each tested category are discussed in detail.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10616 - Entomology

Project

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Continuities

S - Specificky vyzkum na vysokych skolach

Publication year

2024

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

JOURNAL OF MEDICAL ENTOMOLOGY

ISSN

0022-2585

e-ISSN

0022-2585

Volume of the periodical

61

Issue of the periodical within the volume

3

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

13

Pages from-to

541-553

UT code for WoS article

001122470200001

EID of the result in the Scopus database

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