The effect of aldehyde fixatives, alcohol-based fixatives, and fixatives combining denaturation and crosslinking on histomorphological, histochemical, and molecular genetic studies during short-term and long-term fixation: a comprehensive analysis
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F24%3A10486051" target="_blank" >RIV/00216208:11110/24:10486051 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11140/24:10486051 RIV/62157124:16170/24:43881225
Result on the web
<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=UCQ.P.5lPN" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=UCQ.P.5lPN</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.2754/avb202493030321" target="_blank" >10.2754/avb202493030321</a>
Alternative languages
Result language
angličtina
Original language name
The effect of aldehyde fixatives, alcohol-based fixatives, and fixatives combining denaturation and crosslinking on histomorphological, histochemical, and molecular genetic studies during short-term and long-term fixation: a comprehensive analysis
Original language description
The choice of fixative solution has a significant impact on the quality of tissue processing and the results of ancillary pathological studies. The present study aimed to compare the effect of different fixatives and fixative times on routinely used histopathological staining methods and molecular genetic studies in porcine endometrium. Haematoxylin and eosin staining was used to evaluate cell morphology, while Gömöri and orcein staining were selected for histochemical studies; molecular genetic studies were represented by polymerase chain reaction. Tissue samples were fixed in formalin, Bouin's fluid, methacarn, Greenfix, and Antigenfix for 1.5; 8.5; 15.5; 29.5; 64.5; 189.5; 249.5; 309.5; 369.5 days (only the period 1.5 days to 189.5 days was analyzed for histochemical studies). Formol proved to be the best for preserving cell morphology during both short-term and long-term fixation. Alcohol fixatives mainly affected the nuclear morphology, therefore they are not completely suitable for histomorphological analysis. The best preservation of nuclear morphology was achieved with Bouin's fixative. For histochemical studies and shortterm fixation, fixative solutions of all 3 groups (crosslinking, denaturing fixatives and fixatives combining both denaturing and cross-linking effects) were suitable. For long-term fixation, the alcohol fixative methacarn proved to be the best in histochemical studies. Finally, for molecular genetic studies, methacarn was the best fixative, while Bouin's fluid was completely unsuitable. Formalin was confirmed as the most universal fixative overall. However, the selection of a suitable fixative should always be made depending on the specific pathological study and the f ixation time.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30109 - Pathology
Result continuities
Project
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Continuities
V - Vyzkumna aktivita podporovana z jinych verejnych zdroju
Others
Publication year
2024
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Acta Veterinaria Brno
ISSN
0001-7213
e-ISSN
1801-7576
Volume of the periodical
93
Issue of the periodical within the volume
3
Country of publishing house
CZ - CZECH REPUBLIC
Number of pages
10
Pages from-to
321-330
UT code for WoS article
001338130400009
EID of the result in the Scopus database
2-s2.0-85206896623