Different fixative solutions in the detection of mast cells in the canine and feline reproductive organs
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16170%2F20%3A43878751" target="_blank" >RIV/62157124:16170/20:43878751 - isvavai.cz</a>
Alternative codes found
RIV/62157124:16810/20:43878751
Result on the web
<a href="https://journals.viamedica.pl/folia_morphologica/article/view/64408" target="_blank" >https://journals.viamedica.pl/folia_morphologica/article/view/64408</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.5603/FM.a2019.0097" target="_blank" >10.5603/FM.a2019.0097</a>
Alternative languages
Result language
angličtina
Original language name
Different fixative solutions in the detection of mast cells in the canine and feline reproductive organs
Original language description
Background: The aim of the study was to evaluate the usability of different fixative fluids in the detection of mast cells in ovaries and uteri of female dogs and cats. Materials and methods: Samples were fixed in 4% formaldehyde, Carnoy's fluid, Mota's basic lead acetate and isotonic formaldehyde-acetic acid (IFAA). Results: Mast cells (MCs) were detected by acidified toluidine blue staining and counted for various parts of the ovaries and uteri. In the ovaries of both species, the numbers of MCs were significantly (p < 0.05) higher in Carnoy than in formalin. No significant differences were found between Carnoy and Mota (tested only in cats). In the uterus, numbers of MCs were significantly (p < 0.05) higher in Carnoy, Mota and IFAA compared to formalin (canine endometrium, feline endometrium and feline myometrium), in Carnoy and Mota compared to formalin (canine myometrium) and in Mota compared to IFAA (feline myometrium). The majority of MCs were formalin-sensitive in the canine and feline uterus, in the canine ovary and in the feline cortex ovarii. In the feline medulla ovarii, the majority of MCs were formalin-resistant. No formalin-resistant MCs were detected in the feline tunica albuginea ovarii. Conclusions: Thus, using Mota's or Carnoy's fluid in the canine or feline female reproductive organs is recommended. This study improves methodology for all studies which clarify the role of MCs in the reproductive organs of the domestic and laboratory animals.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
40301 - Veterinary science
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Folia morphologica
ISSN
0015-5659
e-ISSN
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Volume of the periodical
79
Issue of the periodical within the volume
2
Country of publishing house
PL - POLAND
Number of pages
7
Pages from-to
265-271
UT code for WoS article
000541424000010
EID of the result in the Scopus database
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