Effect of alcohol-based preservatives on quality of beetle larval specimens
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41330%2F23%3A94105" target="_blank" >RIV/60460709:41330/23:94105 - isvavai.cz</a>
Result on the web
<a href="https://doi.org/10.5593/sgem2022/5.1/s20.019" target="_blank" >https://doi.org/10.5593/sgem2022/5.1/s20.019</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.5593/sgem2022/5.1/s20.019" target="_blank" >10.5593/sgem2022/5.1/s20.019</a>
Alternative languages
Result language
angličtina
Original language name
Effect of alcohol-based preservatives on quality of beetle larval specimens
Original language description
The standard procedure for fluid preservation of insects consists of two steps. First, the specimen is killed and prepared in a fixative fluid and then transferred to a storage fluid. Recommendations for using specific chemicals or mixtures differ depending on the size and anatomy of the animal and vary among the authors. However, controlled comparative studies are often missing. The ideal method should preserve the specimens morphology as well as its genetic information. We have investigated the effect of 12 combinations of commonly used methods based on ethanol, using four types of fixatives (Hot Water Kill (HWK) for 5 minutes/6 hours, absolute ethanol cold/boiling) and three types of storage fluids (70 %, 80 %, and absolute ethanol) after eight months of storage. The effects were tested on mature larvae of a forensically relevant beetle species. We have evaluated the effectivity in terms of color preservation, shape of the specimen, and quality of genetic material. The color was best preserved by HWK for 5 min with subsequent storage in absolute ethanol, and the worst results were achieved by cold absolute ethanol with subsequent storage in 70 % ethanol. The fixative type has a major effect on the shape compared to the storage fluid. The effect of treatments on shape appears to be inverse to the effect on color preservation. The genetic material was preserved equally well in all treatments. The advantages and disadvantages of specific treatments and the recommendation of the best method are discussed.
Czech name
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Czech description
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Classification
Type
D - Article in proceedings
CEP classification
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OECD FORD branch
10616 - Entomology
Result continuities
Project
<a href="/en/project/VI20192022130" target="_blank" >VI20192022130: Utilizing beetle larvae of family Silphidae in forensic practice</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2023
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Article name in the collection
22nd International Multidisciplinary Scientific GeoConference SGEM 2022
ISBN
978-619-7603-46-0
ISSN
1314-2704
e-ISSN
1314-2704
Number of pages
8
Pages from-to
143-150
Publisher name
neuvedeno
Place of publication
Bulgaria
Event location
Albena, Bulharsko
Event date
Jan 1, 2022
Type of event by nationality
WRD - Celosvětová akce
UT code for WoS article
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