Cell-Free Urinary MicroRNAs Expression in Small-Scale Experiments.

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00023698%3A_____%2F17%3AN0000029" target="_blank" >RIV/00023698:_____/17:N0000029 - isvavai.cz</a>

Alternative codes found

RIV/65269705:_____/17:00068075

Result on the web

<a href="https://link.springer.com/protocol/10.1007%2F978-1-4939-6866-4_8" target="_blank" >https://link.springer.com/protocol/10.1007%2F978-1-4939-6866-4_8</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/978-1-4939-6866-4_8" target="_blank" >10.1007/978-1-4939-6866-4_8</a>

Result language

angličtina

Original language name

Cell-Free Urinary MicroRNAs Expression in Small-Scale Experiments.

Original language description

Cell-free microRNAs (miRNAs) have become one of the novel promising diagnostic and prognostic biomarkers for various diseases recently. Blood serum and plasma along with urine are the most common sources of clinically well, almost noninvasively available samples containing various types of miRNAs. Here, we present a protocol for a small-scale study investigating expression of several candidate miRNAs. Small-scale experiments may be worth investigating in cases where no information is available on miRNAs expression in particular diseases, for validation of previously published miRNAs with promising diagnostic potential, particularly in situations where follow-up study is aimed at validating miRNAs coming from array or NGS experiments, or where funding for these large-scale experiments is not available.Using urine miRNAs expression as the novel diagnostic tools is challenging and currently this approach is still in its infancy. Therefore, various methods may result in different conclusions depending on clinical sample sets and differences among methods used for the miRNAs isolation and quantitation. In this protocol, we present the method evaluated in the study focused on cell-free urinary miRNAs in ovarian and endometrial cancers. We recommend using stabilization tubes for the urine collection, as this step may be necessary to stop activity of RNases. Further, routine real-time PCR methods are described. We demonstrate that assessment of urinary miRNAs expression may reveal as a feasible method to explore the potential for finding novel diagnostic and prognostic markers.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10608 - Biochemistry and molecular biology

Project

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Continuities

V - Vyzkumna aktivita podporovana z jinych verejnych zdroju

Publication year

2017

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Methods in Molecular Biology

ISSN

1064-3745

e-ISSN

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Volume of the periodical

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Issue of the periodical within the volume

1580

Country of publishing house

GB - UNITED KINGDOM

Number of pages

8

Pages from-to

99-106

UT code for WoS article

999

EID of the result in the Scopus database

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