High miR-451 expression in peripheral blood mononuclear cells from subjects at risk of developing rheumatoid arthritis

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00023728%3A_____%2F21%3AN0000029" target="_blank" >RIV/00023728:_____/21:N0000029 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11110/21:10427988 RIV/00216208:11310/21:10427988

Result on the web

<a href="https://doi.org/10.1038/s41598-021-84004-3" target="_blank" >https://doi.org/10.1038/s41598-021-84004-3</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1038/s41598-021-84004-3" target="_blank" >10.1038/s41598-021-84004-3</a>

Result language

angličtina

Original language name

High miR-451 expression in peripheral blood mononuclear cells from subjects at risk of developing rheumatoid arthritis

Original language description

Individuals carrying anti-citrullinated protein antibodies (ACPA) are considered at high risk of developing rheumatoid arthritis (RA). The altered expression of miRNAs contributes to the pathogenesis of RA. We aimed to identify differentially expressed miRNAs in the peripheral blood of ACPA-positive individuals with arthralgia at risk of RA compared to healthy controls (HC) and to determine their implications in the preclinical phase of RA. A comprehensive analysis of miRNAs revealed the dysregulation of miR-451 in peripheral blood mononuclear cells (PBMC) and plasma from RA-risk individuals. Higher miR-451 expression in PBMC from RA-risk individuals was further validated. Notably, miR-451 was previously shown to regulate CXCL16, a protein involved in RA pathogenesis. The expression of miR-451 in PBMC positively correlated with the CXCL16 mRNA, which could be secondary to the inflammation-induced expression of miR-451. Transfection of monocytes with pre-miR-451 in vitro resulted in the downregulation of CXCL16. Moreover, flow cytometry revealed a lower count of CXCL16-positive monocytes in RA-risk individuals. We propose that the constitutive or inflammation-induced upregulation of miR-451 in PBMC downregulates the expression of CXCL16, reduces the inflammatory milieu and thereby strives to delay the shift from the preclinical phase to the clinical manifestation of RA. This hypothesis warrants further investigation.

Czech name

—

Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

30226 - Rheumatology

Project

<a href="/en/project/NV17-32612A" target="_blank" >NV17-32612A: Clinically suspect arhralgia: characteristics of the preclinical phase of rheumatoid arthritis</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2021

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

SCIENTIFIC REPORTS

ISSN

2045-2322

e-ISSN

—

Volume of the periodical

11

Issue of the periodical within the volume

Art. Nr. 4719

Country of publishing house

DE - GERMANY

Number of pages

9

Pages from-to

1-9

UT code for WoS article

000626621800079

EID of the result in the Scopus database

2-s2.0-85101570912