Unraveling the complexity of tyrosine kinase inhibitor-resistant populations by ultra-deep sequencing of the BCR-ABL kinase domain
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00023736%3A_____%2F13%3A00010715" target="_blank" >RIV/00023736:_____/13:00010715 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1182/blood-2013-03-487728" target="_blank" >http://dx.doi.org/10.1182/blood-2013-03-487728</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1182/blood-2013-03-487728" target="_blank" >10.1182/blood-2013-03-487728</a>
Alternative languages
Result language
angličtina
Original language name
Unraveling the complexity of tyrosine kinase inhibitor-resistant populations by ultra-deep sequencing of the BCR-ABL kinase domain
Original language description
We found that conventional Sanger sequencing had misclassified or underestimated BCR-ABL mutation status in 55% of the samples, where mutations with 1-15% abundance were detected. A complex clonal texture was uncovered by clonal analysis of samples harbouring multiple mutations and up to thirteen different mutated populations were identified. The landscape of these mutated populations was found to be highly dynamic. The high degree of complexity uncovered by UDS indicates that conventional Sanger sequencing might be an inadequate tool to assess BCR-ABL KD mutation status, that currently represents an important component of the therapeutic decision algorithms. Further evaluation of the clinical usefulness of UDS-based approaches is warranted.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
FD - Oncology and haematology
OECD FORD branch
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Result continuities
Project
<a href="/en/project/NT11555" target="_blank" >NT11555: DNA fingerprinting in chronic myeloid leukemia</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2013
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Blood
ISSN
0006-4971
e-ISSN
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Volume of the periodical
122
Issue of the periodical within the volume
9
Country of publishing house
US - UNITED STATES
Number of pages
14
Pages from-to
1634-1648
UT code for WoS article
000324056500018
EID of the result in the Scopus database
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