Lifetime-based photoconversion of EGFP as a tool for FLIM
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00023736%3A_____%2F19%3A00012442" target="_blank" >RIV/00023736:_____/19:00012442 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11320/19:10386976
Result on the web
<a href="https://doi.org/10.1016/j.bbagen.2018.10.016" target="_blank" >https://doi.org/10.1016/j.bbagen.2018.10.016</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.bbagen.2018.10.016" target="_blank" >10.1016/j.bbagen.2018.10.016</a>
Alternative languages
Result language
angličtina
Original language name
Lifetime-based photoconversion of EGFP as a tool for FLIM
Original language description
We report on so far unexplored lifetime photoconversion of EGFP usable in FLIM. Fluorescence lifetime imaging and spectral FLIM has been used for characterization of the EGFP photoconversion and protein tracking. Besides potential FLIM artifacts caused by an unintended EGFP photoconversion, the controlled photoconversion turns EGFP to an excellent tool for kinetic FLIM applications. Since the photoconversion occurs in the lifetime domain, PC-EGFP can be easily distinguished from the unconverted tag by time-resolved detection while all other spectral channels stay free for multicolor labeling. The reported lifetime photoconversion lines up EGFP with other photoconvertible fluorescent proteins with special advantage for fluorescence lifetime imaging where lifetime-photoconvertible labels are scarce.
Czech name
—
Czech description
—
Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
30205 - Hematology
Result continuities
Project
<a href="/en/project/ED4.1.00%2F16.0340" target="_blank" >ED4.1.00/16.0340: Upgrade of facilities for physical research and education</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Biochimica et biophysica acta: general subjects
ISSN
0304-4165
e-ISSN
—
Volume of the periodical
1863
Issue of the periodical within the volume
1
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
12
Pages from-to
266-277
UT code for WoS article
000452343100025
EID of the result in the Scopus database
2-s2.0-85055820732