Two-photon lifetime-based photoconversion of EGFP for 3D-photostimulation in FLIM
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00023736%3A_____%2F23%3A00013552" target="_blank" >RIV/00023736:_____/23:00013552 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11320/23:10467463
Result on the web
<a href="https://doi.org/10.1088/2050-6120/acdb31" target="_blank" >https://doi.org/10.1088/2050-6120/acdb31</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1088/2050-6120/acdb31" target="_blank" >10.1088/2050-6120/acdb31</a>
Alternative languages
Result language
angličtina
Original language name
Two-photon lifetime-based photoconversion of EGFP for 3D-photostimulation in FLIM
Original language description
Enhanced green fluorescence protein (EGFP) is a fluorescent tag commonly used in cellular and biomedical applications. Surprisingly, some interesting photochemical properties of EGFP have remained unexplored. Here we report on two-photon-induced photoconversion of EGFP, which can be permanently converted by intense IR irradiation to a form with a short fluorescence lifetime and spectrally conserved emission. Photoconverted EGFP thus can be distinguished from the unconverted tag by the time-resolved detection. Nonlinear dependence of the two-photon photoconversion efficiency on the light intensity allows for an accurate 3D localization of the photoconverted volume within cellular structures, which is especially useful for kinetic FLIM applications.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30205 - Hematology
Result continuities
Project
<a href="/en/project/GA22-03875S" target="_blank" >GA22-03875S: Role of acute myeloid leukemia-associated nucleophosmin mutations in the NPM-p53-Mdm2 regulatory network</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2023
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Methods and applications in fluorescence
ISSN
2050-6120
e-ISSN
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Volume of the periodical
11
Issue of the periodical within the volume
3
Country of publishing house
GB - UNITED KINGDOM
Number of pages
13
Pages from-to
"art. no. 034002"
UT code for WoS article
001003431600001
EID of the result in the Scopus database
2-s2.0-85162096822