Digital PCR can provide improved BCR-ABL1 detection in chronic myeloid leukemia patients in deep molecular response and sensitivity of standard quantitative methods using EAC assays
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00023736%3A_____%2F21%3A00013224" target="_blank" >RIV/00023736:_____/21:00013224 - isvavai.cz</a>
Alternative codes found
RIV/65269705:_____/21:00074218 RIV/00216224:14740/21:00120108
Result on the web
<a href="https://doi.org/10.1016/j.plabm.2021.e00210" target="_blank" >https://doi.org/10.1016/j.plabm.2021.e00210</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.plabm.2021.e00210" target="_blank" >10.1016/j.plabm.2021.e00210</a>
Alternative languages
Result language
angličtina
Original language name
Digital PCR can provide improved BCR-ABL1 detection in chronic myeloid leukemia patients in deep molecular response and sensitivity of standard quantitative methods using EAC assays
Original language description
BCR-ABL1 molecular detection using quantitative PCR (qPCR) methods is the golden standard of chronic myeloid leukemia (CML) monitoring. However, due to variable sensitivity of qPCR assays across laboratories, alternative methods are tested. Digital PCR (dPCR) has been suggested as a robust and reproducible option. Here we present a comparison of droplet dPCR with routinely used reverse-transcription qPCR (RT-qPCR) and automated GeneXpert systems. Detection limit of dPCR was above 3 BCR-ABL1 copies, although due to background amplification the resulting sensitivity was 0.01% BCR-ABL1 (MR4.0). Nevertheless, in comparison with GeneXpert, dPCR categorized more than 50% of the patients into different MR groups, showing a potential for improved BCR-ABL1 detection.
Czech name
—
Czech description
—
Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
30205 - Hematology
Result continuities
Project
<a href="/en/project/NV17-30397A" target="_blank" >NV17-30397A: Mutational screening of primitive cell populations in chronic myeloid leukemia: BCR-ABL1 and beyond</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Practical laboratory medicine
ISSN
2352-5517
e-ISSN
—
Volume of the periodical
25
Issue of the periodical within the volume
[May]
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
6
Pages from-to
"art. no. e00210"
UT code for WoS article
000653682600011
EID of the result in the Scopus database
2-s2.0-85102511335