Digital PCR Applications in the SARS-CoV-2/COVID-19 Era: a Roadmap for Future Outbreaks
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26220%2F22%3APU148230" target="_blank" >RIV/00216305:26220/22:PU148230 - isvavai.cz</a>
Result on the web
<a href="https://journals.asm.org/doi/full/10.1128/cmr.00168-21" target="_blank" >https://journals.asm.org/doi/full/10.1128/cmr.00168-21</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1128/cmr.00168-21" target="_blank" >10.1128/cmr.00168-21</a>
Alternative languages
Result language
angličtina
Original language name
Digital PCR Applications in the SARS-CoV-2/COVID-19 Era: a Roadmap for Future Outbreaks
Original language description
The ongoing coronavirus disease 2019 (COVID-19) pandemic, caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has led to a global public health disaster. The current gold standard for the diagnosis of infected patients is real-time reverse transcription-quantitative PCR (RT-qPCR). As effective as this method may be, it is subject to false-negative and -positive results, affecting its precision, especially for the detection of low viral loads in samples. In contrast, digital PCR (dPCR), the third generation of PCR, has been shown to be more effective than the gold standard, RT-qPCR, in detecting low viral loads in samples. In this review article, we selected publications to show the broad-spectrum applications of dPCR, including the development of assays and reference standards, environmental monitoring, mutation detection, and clinical diagnosis of SARS-CoV-2, while comparing it analytically to the gold standard, RT-qPCR. In summary, it is evident that the specificity, sensitivity, reproducibility, and detection limits of RT-dPCR are generally unaffected by common factors that may affect RT-qPCR. As this is the first time that dPCR is being tested in an outbreak of such a magnitude, knowledge of its applications will help chart a course for future diagnosis and monitoring of infectious disease outbreaks.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30303 - Infectious Diseases
Result continuities
Project
<a href="/en/project/VI04000057" target="_blank" >VI04000057: An ultrafast portable system to detect SARS-CoV-2</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Clinical Microbiology Reviews
ISSN
0893-8512
e-ISSN
1098-6618
Volume of the periodical
35
Issue of the periodical within the volume
3
Country of publishing house
US - UNITED STATES
Number of pages
37
Pages from-to
„“-„“
UT code for WoS article
000856911400002
EID of the result in the Scopus database
2-s2.0-85138460062