Human myeloid-derived suppressor cell expansion during sepsis is revealed by unsupervised clustering of flow cytometric data
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00023736%3A_____%2F21%3A00013236" target="_blank" >RIV/00023736:_____/21:00013236 - isvavai.cz</a>
Alternative codes found
RIV/00159816:_____/21:00075205 RIV/00216224:14110/21:00124029
Result on the web
<a href="https://doi.org/10.1002/eji.202049141" target="_blank" >https://doi.org/10.1002/eji.202049141</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/eji.202049141" target="_blank" >10.1002/eji.202049141</a>
Alternative languages
Result language
angličtina
Original language name
Human myeloid-derived suppressor cell expansion during sepsis is revealed by unsupervised clustering of flow cytometric data
Original language description
Myeloid-derived suppressor cells (MDSCs) are important regulators of immune processes during sepsis in mice. However, confirming these observations in humans has been challenging due to the lack of defined preparation protocols and phenotyping schemes for MDSC subsets. Thus, it remains unclear how MDSCs are involved in acute sepsis and whether they have a role in the long-term complications seen in survivors. Here, we combined comprehensive flow cytometry phenotyping with unsupervised clustering using self-organizing maps to identify the three recently defined human MDSC subsets in blood from severe sepsis patients, long-term sepsis survivors, and age-matched controls. We demonstrated the expansion of monocytic M-MDSCs and polymorphonuclear PMN-MDSCs, but not early-stage (e)-MDSCs during acute sepsis. High levels of PMN-MDSCs were also present in long-term survivors many months after discharge, suggesting a possible role in sepsis-related complications. Altogether, by employing unsupervised clustering of flow cytometric data we have confirmed the likely involvement of human MDSC subsets in acute sepsis, and revealed their expansion in sepsis survivors at late time points. The application of this strategy in future studies and in the clinical/diagnostic context would enable rapid progress toward a full understanding of the roles of MDSC in sepsis and other inflammatory conditions.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30205 - Hematology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
European journal of immunology
ISSN
0014-2980
e-ISSN
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Volume of the periodical
51
Issue of the periodical within the volume
7
Country of publishing house
US - UNITED STATES
Number of pages
7
Pages from-to
1785-1791
UT code for WoS article
000651239400001
EID of the result in the Scopus database
2-s2.0-85104564654