Human decellularized and crosslinked pericardium coated with bioactive molecular assemblies

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00023752%3A_____%2F20%3A43920010" target="_blank" >RIV/00023752:_____/20:43920010 - isvavai.cz</a>

Result on the web

<a href="https://iopscience.iop.org/article/10.1088/1748-605X/ab52db" target="_blank" >https://iopscience.iop.org/article/10.1088/1748-605X/ab52db</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1088/1748-605X/ab52db" target="_blank" >10.1088/1748-605X/ab52db</a>

Result language

angličtina

Original language name

Human decellularized and crosslinked pericardium coated with bioactive molecular assemblies

Original language description

Decellularized human pericardium is under study as an allogenic material for cardiovascular application purposes. The effects of crosslinking on the mechanical properties of decellularized pericardium were determined via a uniaxial tensile test, and the effects of crosslinking on the collagen structure of decellularized pericardium were determined via multiphoton microscopy. The viability of human umbilical vein endothelial cells seeded on decellularized human pericardium and on pericardium strongly and weakly crosslinked with glutaraldehyde and with genipin were evaluated by means of an MTS assay. The viability of the cells measured via their metabolic activity decreased considerably when the pericardium was crosslinked with glutaraldehyde. Conversely, the cell viability increased when the pericardium was crosslinked with genipin. Coating both non-modified pericardium and crosslinked pericardium with a fibrin mesh or with a mesh containing attached heparin and/or fibronectin led to a significant increase in cell viability. The highest degree of viability was attained for samples that were weakly crosslinked with genipin and modified by means of a fibrin and fibronectin coating. The results indicate a way by which in vivo endothelialization of human cardiac allografts or xenografts could potentially be encouraged.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

30201 - Cardiac and Cardiovascular systems

Project

—

Continuities

V - Vyzkumna aktivita podporovana z jinych verejnych zdroju

Publication year

2020

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Biomedical Materials

ISSN

1748-605X

e-ISSN

—

Volume of the periodical

15

Issue of the periodical within the volume

1

Country of publishing house

GB - UNITED KINGDOM

Number of pages

10

Pages from-to

"Article number 015008"

UT code for WoS article

000563778900006

EID of the result in the Scopus database

2-s2.0-85076327091