Neonatal Clonazepam Administration Induced Long-Lasting Changes in GABA(A) and GABA(B) Receptors

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00023752%3A_____%2F20%3A43920369" target="_blank" >RIV/00023752:_____/20:43920369 - isvavai.cz</a>

Result on the web

<a href="https://www.mdpi.com/1422-0067/21/9/3184" target="_blank" >https://www.mdpi.com/1422-0067/21/9/3184</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/ijms21093184" target="_blank" >10.3390/ijms21093184</a>

Result language

angličtina

Original language name

Neonatal Clonazepam Administration Induced Long-Lasting Changes in GABA(A) and GABA(B) Receptors

Original language description

Benzodiazepines (BZDs) are widely used in patients of all ages. Unlike adults, neonatal animals treated with BZDs exhibit a variety of behavioral deficits later in life; however, the mechanisms underlying these deficits are poorly understood. This study aims to examine whether administration of clonazepam (CZP; 1 mg/kg/day) in 7–11-day-old rats affects Gama aminobutyric acid (GABA)ergic receptors in both the short and long terms. Using RT-PCR and quantitative autoradiography, we examined the expression of the selected GABAA receptor subunits (α1, α2, α4, γ2, and δ) and the GABAB B2 subunit, and GABAA, benzodiazepine, and GABAB receptor binding 48 h, 1 week, and 2 months after treatment discontinuation. Within one week after CZP cessation, the expression of the α2 subunit was upregulated, whereas that of the δ subunit was downregulated in both the hippocampus and cortex. In the hippocampus, the α4 subunit was downregulated after the 2-month interval. Changes in receptor binding were highly dependent on the receptor type, the interval after treatment cessation, and the brain structure. GABAA receptor binding was increased in almost all of the brain structures after the 48-h interval. BZD-binding was decreased in many brain structures involved in the neuronal networks associated with emotional behavior, anxiety, and cognitive functions after the 2-month interval. Binding of the GABAB receptors changed depending on the interval and brain structure. Overall, the described changes may affect both synaptic development and functioning and may potentially cause behavioral impairment.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

30103 - Neurosciences (including psychophysiology)

Project

<a href="/en/project/EF16_025%2F0007444" target="_blank" >EF16_025/0007444: PharmaBrain</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2020

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

International Journal of Molecular Sciences

ISSN

1661-6596

e-ISSN

—

Volume of the periodical

21

Issue of the periodical within the volume

9

Country of publishing house

CH - SWITZERLAND

Number of pages

20

Pages from-to

3184

UT code for WoS article

000535581700155

EID of the result in the Scopus database

2-s2.0-85084276453