Stat3 Silencing Affects Circadian Clock Gene Expression and Lipopolysaccharide Response in the Suprachiasmatic Nucleus, Cortex, and Glioblastoma Cell Cultures
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00023752%3A_____%2F25%3A43921512" target="_blank" >RIV/00023752:_____/25:43921512 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11120/25:43928413 RIV/00216208:11310/25:10497865
Result on the web
<a href="https://doi.org/10.1096/fj.202403177RR" target="_blank" >https://doi.org/10.1096/fj.202403177RR</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1096/fj.202403177RR" target="_blank" >10.1096/fj.202403177RR</a>
Alternative languages
Result language
angličtina
Original language name
Stat3 Silencing Affects Circadian Clock Gene Expression and Lipopolysaccharide Response in the Suprachiasmatic Nucleus, Cortex, and Glioblastoma Cell Cultures
Original language description
In mammals, the suprachiasmatic nucleus (SCN) serves as the central circadian pacemaker, regulating rhythms essential for physiological processes. STAT3, a transcription factor primarily involved in immune signaling, exhibits circadian rhythmicity in SCN astrocytes. This study examined the role of STAT3 in circadian regulation across several cell types, including primary cultures of rat SCN and cortex, SCN cells and organotypic SCN slices from PER2::LUC mice, and C6 glioblastoma cells. Furthermore, the involvement of STAT3 in inflammatory responses was investigated in SCN and cortical primary cultures. STAT3 silencing enhanced Bmal1 expression across all tested cell types, disrupted Bmal1 rhythmicity in C6 cells, and reduced the amplitude of the PER2-driven rhythm in bioluminescence in SCN primary cells and organotypic cultures. In SCN cells, STAT3 silencing also attenuated its own expression and Gfap, whereas in cortical cells, it exhibited broader effects. Under LPS stimulation, STAT3 silencing in SCN cells reduced most LPS-induced genes, including inflammatory and oxidative stress markers, while showing variable effects in cortical cells. These findings indicate that while the role of STAT3 in the circadian clockwork appears consistent across cell types, its involvement in functional gene expression and immune responses may vary depending on the tissue and differ between SCN and cortical primary cells.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10601 - Cell biology
Result continuities
Project
<a href="/en/project/LX22NPO5107" target="_blank" >LX22NPO5107: National institute for Neurological Research</a><br>
Continuities
V - Vyzkumna aktivita podporovana z jinych verejnych zdroju
Others
Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
The FASEB Journal
ISSN
0892-6638
e-ISSN
1530-6860
Volume of the periodical
39
Issue of the periodical within the volume
10
Country of publishing house
US - UNITED STATES
Number of pages
12
Pages from-to
"Article number e70577"
UT code for WoS article
001487870800001
EID of the result in the Scopus database
2-s2.0-105005219702