Development of Competitive ELISAs Suitable for Detection of Recombinant Human Growth Hormone in Illegal Nutritional Supplements
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00023752%3A_____%2F25%3A43921557" target="_blank" >RIV/00023752:_____/25:43921557 - isvavai.cz</a>
Alternative codes found
RIV/60461373:22330/25:43932637 RIV/60461373:22340/25:43932637
Result on the web
<a href="https://onlinelibrary.wiley.com/doi/10.1155/jamc/3021570" target="_blank" >https://onlinelibrary.wiley.com/doi/10.1155/jamc/3021570</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1155/jamc/3021570" target="_blank" >10.1155/jamc/3021570</a>
Alternative languages
Result language
angličtina
Original language name
Development of Competitive ELISAs Suitable for Detection of Recombinant Human Growth Hormone in Illegal Nutritional Supplements
Original language description
The use of human growth hormone (hGH) by athletes remains widespread despite being banned by the World Anti-Doping Agency (WADA). Current commercial ELISA kits for the determination of hGH are predominantly based on the sandwich format of this method, which allows the analysis of the whole hGH molecules, but not their fragments. The abuse of these fragments by athletes is the current trend in doping. Therefore, a competitive ELISA format is needed; however, only a limited number of kits on the market are based on this principle. Furthermore, these kits are designed to detect and quantify hGH only in blood plasma, serum or tissue cultures. When analysing a nutritional supplement containing hGH using one of these ELISA kits, a false negative result was observed. In the present work, two competitive ELISA methods for the detection of hGH in illegal nutritional supplements were developed. The development of the methods involved the selection of suitable immunoreagents and their combinations with subsequent optimisation of the conditions for their use. The methods were then characterised (with LOD 0.4 and 18.4 ng/mL, according to the primary antibody) and applied to analyse real nutritional supplements. In all 34 samples suspected of containing hGH, the hormone was detected using both ELISA methods and subsequently confirmed by LC-UV and LC-MS. Therefore, the newly developed ELISA methods could become a valuable tool for the police in identifying illegal preparations.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10401 - Organic chemistry
Result continuities
Project
<a href="/en/project/VJ01010043" target="_blank" >VJ01010043: Pharmacrime – forensic identification of prohormones and counterfeid medicine</a><br>
Continuities
V - Vyzkumna aktivita podporovana z jinych verejnych zdroju
Others
Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Analytical Methods in Chemistry
ISSN
2090-8865
e-ISSN
2090-8873
Volume of the periodical
2025
Issue of the periodical within the volume
1
Country of publishing house
GB - UNITED KINGDOM
Number of pages
13
Pages from-to
"Article Number 3021570"
UT code for WoS article
001503815900001
EID of the result in the Scopus database
2-s2.0-105007645115