Immunochemical techniques for anabolic androgenic steroid – matrix effects study for food supplements
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F19%3A43919744" target="_blank" >RIV/60461373:22330/19:43919744 - isvavai.cz</a>
Alternative codes found
RIV/60461373:22810/19:43919744
Result on the web
<a href="https://link.springer.com/article/10.1007%2Fs00217-018-3204-3" target="_blank" >https://link.springer.com/article/10.1007%2Fs00217-018-3204-3</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s00217-018-3204-3" target="_blank" >10.1007/s00217-018-3204-3</a>
Alternative languages
Result language
angličtina
Original language name
Immunochemical techniques for anabolic androgenic steroid – matrix effects study for food supplements
Original language description
Anabolic androgenic steroid contaminations in food supplements are common, and their consumption may lead to positive doping results. One of such anabolic steroid is mesterolone selected as an analyte for the present study. Some contaminated supplements cannot be detected by traditional methods due to the matrix effect. Two variants of indirect competitive immunochemical methodan enzyme-linked immunosorbent assay and an immunochromatographic method were developed using rabbit polyclonal antibodies and corresponding mesterolone conjugated at position C-17 to bovine serum albumin. Under optimal experimental conditions, the ELISA achieved the limit of the detection value of 0.010 +/- 0.003ngmL(-1) and for LFIA visual detection limit was determined as concentration 50ngmL(-1). Both methods showed the mesterolone specificity of anti-mesterolone antibodies. The developed ELISA and LFIA were then applied to artificially contaminated food supplements. Before immunoassays, the samples were treated using a simple extraction protocol that did not include preliminary clean-up steps. For ELISA, a negative effect on the sensitivity of the method was observed for complex matrix samples. All type of analysed sample matrixes have minimal effect on LFIA sensitivity and, therefore, it could be successfully applied for the simple screening quality of food supplements.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10406 - Analytical chemistry
Result continuities
Project
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Continuities
S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
European Food Research and Technology
ISSN
1438-2377
e-ISSN
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Volume of the periodical
245
Issue of the periodical within the volume
5
Country of publishing house
DE - GERMANY
Number of pages
9
Pages from-to
1011-1019
UT code for WoS article
000467903900006
EID of the result in the Scopus database
2-s2.0-85057232767