Rapid immunoassay for the detection of anabolic steroid methandienone
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F14%3A43899085" target="_blank" >RIV/60461373:22330/14:43899085 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Rapid immunoassay for the detection of anabolic steroid methandienone
Original language description
Methandienone, sometimes known as Dianabol is one of the most commonly abused anabolic steroid. The aim of this work is to develop an indirect competitive Enzyme Linked ImmunoSorbent Assay (ELISA) for the detection of methandienone in food supplements. Under the optimal experimental conditions, the most sensitive assay achieved IC50 and the limit of the detection the values of 1.45 ng/mL and 0.17 ng/mL, respectively. Specificity was verified by the testing of the cross-reactivity. The reactivity was examined by 44 standards of anabolic steroids. Furthermore, the different matrices of dietary supplements and sample preparation were tested and finally the method was successfully applied to real samples.
Czech name
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Czech description
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Classification
Type
O - Miscellaneous
CEP classification
GM - Food industry
OECD FORD branch
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Result continuities
Project
<a href="/en/project/VG20112015045" target="_blank" >VG20112015045: Rapid immunochemical tests for the detection of steroidal anabolics</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů