Development of immunochemical method for the methandienone detection
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F14%3A43899088" target="_blank" >RIV/60461373:22330/14:43899088 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Development of immunochemical method for the methandienone detection
Original language description
Methandienone, 17beta-hydroxy-17alfa-methylandrosta-1,4-dien-3-one, also known as DianabolTM is a synthetic exogenous steroid derived from testosterone. The aim of this work was to develop two formats of immunochromatographic test for the detection of anabolic steroid methandienone in nutritional supplements based on methandienone haptens which were conjugated in position C3 and C17 of steroid skeleton. Suitable conditions (concentration of immunoreagents, type of membrane and reaction buffer) were selected, six points calibration curves were prepared and visual detection limits were obtained (1 mýg/mL and 5 mýg/mL for MD-3-CMO-OVA and for the MD-17-HS-OVA system, respectively). In real samples of food supplements, both systems successful detected methandienone and the similar steroid.
Czech name
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Czech description
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Classification
Type
O - Miscellaneous
CEP classification
CB - Analytical chemistry, separation
OECD FORD branch
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Result continuities
Project
<a href="/en/project/VG20112015045" target="_blank" >VG20112015045: Rapid immunochemical tests for the detection of steroidal anabolics</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů