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Development of immunochemical method for the methandienone detection

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F14%3A43899088" target="_blank" >RIV/60461373:22330/14:43899088 - isvavai.cz</a>

  • Result on the web

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    Development of immunochemical method for the methandienone detection

  • Original language description

    Methandienone, 17beta-hydroxy-17alfa-methylandrosta-1,4-dien-3-one, also known as DianabolTM is a synthetic exogenous steroid derived from testosterone. The aim of this work was to develop two formats of immunochromatographic test for the detection of anabolic steroid methandienone in nutritional supplements based on methandienone haptens which were conjugated in position C3 and C17 of steroid skeleton. Suitable conditions (concentration of immunoreagents, type of membrane and reaction buffer) were selected, six points calibration curves were prepared and visual detection limits were obtained (1 mýg/mL and 5 mýg/mL for MD-3-CMO-OVA and for the MD-17-HS-OVA system, respectively). In real samples of food supplements, both systems successful detected methandienone and the similar steroid.

  • Czech name

  • Czech description

Classification

  • Type

    O - Miscellaneous

  • CEP classification

    CB - Analytical chemistry, separation

  • OECD FORD branch

Result continuities

  • Project

    <a href="/en/project/VG20112015045" target="_blank" >VG20112015045: Rapid immunochemical tests for the detection of steroidal anabolics</a><br>

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Others

  • Publication year

    2014

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů